ArticleProceedings of the National Academy of Sciences of the United States of America1998
Identification of thyroid hormone response elements in the human fatty acid synthase promoter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 45 citations in OpenAlex.
- Understanding the Roles of Selenium on Thyroid Hormone-Induced Thermogenesis in Adipose Tissue.Biological trace element research · 2024Review
- A Transgenic Mouse Model for Detection of Tissue-Specific Thyroid Hormone Action.Endocrinology · 2018Article
- The thyroid hormone-αvβ3 integrin axis in ovarian cancer: regulation of gene transcription and MAPK-dependent proliferation.Oncogene · 2016Article
- Genetic evidence that thyroid hormone is indispensable for prepubertal insulin-like growth factor-I expression and bone acquisition in mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
- Influence of neonatal hypothyroidism on hepatic gene expression and lipid metabolism in adulthood.PloS one · 2012Article
- Effects of farnesoid X receptor on the expression of the fatty acid synthetase and hepatic lipase.Molecular biology reports · 2011Article
- Multi-tissue gene-expression analysis in a mouse model of thyroid hormone resistance.Genome biology · 2004Article
- Human acetyl-CoA carboxylase 1 gene: presence of three promoters and heterogeneity at the 5'-untranslated mRNA region.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Thyroid Hormone Stimulates Renin Gene Expression Through the Thyroid Hormone Response Element.Hypertension (Dallas, Tex. : 1979) · 2001Article
- Sterol regulation of human fatty acid synthase promoter I requires nuclear factor-Y- and Sp-1-binding sites.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the regulation of the human fatty acid synthase gene by the thyroid hormone triiodothyronine, various constructs of the human fatty acid synthase promoter and the luciferase reporter gene were transfected in combination with plasmids expressing the thyroid hormone and the retinoid X receptors in HepG2 cells. The reporter gene was activated 25-fold by the thyroid hormone in the presence of the thyroid hormone receptor. When both the thyroid hormone and the retinoid X receptors were expressed in HepG2 cells, there was about a 100-fold increase in reporter gene expression. 5'-Deletion analysis disclosed two thyroid hormone response elements, TRE1 (nucleotides -870 to -650) and TRE2 (nucleotides -272 to -40), in the human fatty acid synthase promoter. The presence of thyroid hormone response elements in these two regions of the promoter was confirmed by cloning various fragments of these two regions in the minimal thymidine kinase promoter-luciferase reporter gene plasmid construct and determining reporter gene expression. The results of this cloning procedure and those of electrophoretic mobility shift assays indicated that the sequence GGGTTAcgtcCGGTCA (nucleotides -716 to -731) represents TRE1 and that the sequence GGGTCC (nucleotides -117 to -112) represents TRE2. The sequence of TRE1 is very similar to the consensus sequence of the thyroid hormone response element, whereas the sequence of TRE2 contains only a half-site of the thyroid hormone response element consensus motif because it lacks the direct repeat. The sequences on either side of TRE2 seem to influence its response to the thyroid hormone and retinoid X receptors.
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