ArticleProceedings of the National Academy of Sciences of the United States of America1998
Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 134 citations in OpenAlex.
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic research · 2020Article
- High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane.Frontiers in bioengineering and biotechnology · 2020Article
- Genome-Scale Sequence Disruption Following Biolistic Transformation in Rice and Maize.The Plant cell · 2019Article
- CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects.Plant cell reports · 2016Review
- The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat.Transgenic research · 2015Article
- Genetic and genomic toolbox of Zea mays.Genetics · 2015Review
- Epigenetic silencing in transgenic plants.Frontiers in plant science · 2015Review
- Phospholipid and triacylglycerol profiles modified by PLD suppression in soybean seed.Plant biotechnology journal · 2011Article
- Dosage-dependent gene expression from direct repeat locus in rice developed by site-specific gene integration.Molecular biotechnology · 2010Article
- Quantitative evaluation of six different viral suppressors of silencing using image analysis of transient GFP expression.Plant cell reports · 2009Article
- Agrobacterium-mediated transformation of oat (Avena sativa L.) cultivars via immature embryo and leaf explants.Plant cell reports · 2008Article
- Characterisation of 3' transgene insertion site and derived mRNAs in MON810 YieldGard maize.Plant molecular biology · 2008Article
- Variable T-DNA linkage configuration affects inheritance of carotenogenic transgenes and carotenoid accumulation in transgenic indica rice.Plant cell reports · 2007Article
- Transgene constructs in coho salmon (Oncorhynchus kisutch) are repeated in a head-to-tail fashion and can be integrated adjacent to horizontally-transmitted parasite DNA.Transgenic research · 2006Article
- In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research.Plant methods · 2006Article
- The Quest to Understand the Basis and Mechanisms that Control Expression of Introduced Transgenes in Crop Plants.Plant signaling & behavior · 2006Article
- The mutational consequences of plant transformation.Journal of biomedicine & biotechnology · 2006Article
- A rapid and efficient transformation protocol for the grass Brachypodium distachyon.Plant cell reports · 2005Article
- Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability.Plant molecular biology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integration of transgenic DNA into the plant genome was investigated in 13 transgenic oat (Avena sativa L.) lines produced using microprojectile bombardment with one or two cotransformed plasmids. In all transformation events, the transgenic DNA integrated into the plant genome consisted of intact transgene copies that were accompanied by multiple, rearranged, and/or truncated transgene fragments. All fragments of transgenic DNA cosegregated, indicating that they were integrated at single gene loci. Analysis of the structure of the transgenic loci indicated that the transgenic DNA was interspersed by the host genomic DNA. The number of insertions of transgenic DNA within the transgene loci varied from 2 to 12 among the 13 lines. Restriction endonucleases that do not cleave the introduced plasmids produced restriction fragments ranging from 3.6 to about 60 kb in length hybridizing to a probe comprising the introduced plasmids. Although the size of the interspersing host DNA within the transgene locus is unknown, the sizes of the transgene-hybridizing restriction fragments indicated that the entire transgene locus must be at least from 35-280 kb. The observation that all transgenic lines analyzed exhibited genomic interspersion of multiple clustered transgenes suggests a predominating integration mechanism. We propose that transgene integration at multiple clustered DNA replication forks could account for the observed interspersion of transgenic DNA with host genomic DNA within transgenic loci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.