ArticleRNA (New York, N.Y.)1998
Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 40 citations in OpenAlex.
- Functional and Biochemical Characterization of Dib1's Role in Pre-Messenger RNA Splicing.Journal of molecular biology · 2018Article
- Dramatically reduced spliceosome in Cyanidioschyzon merolae.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Structural studies of the spliceosome: zooming into the heart of the machine.Current opinion in structural biology · 2014Review
- The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.Journal of cellular biochemistry · 2013Article
- Article
- Ribonucleoprotein multimers and their functions.Critical reviews in biochemistry and molecular biology · 2010Review
- Article
- Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.RNA (New York, N.Y.) · 2009Article
- Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.Molecular bioSystems · 2009Review
- Structure and function of an RNase H domain at the heart of the spliceosome.The EMBO journal · 2008Article
- The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing.Nucleic acids research · 2008Article
- The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase.Molecular cell · 2006Article
- Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.RNA (New York, N.Y.) · 2006Article
- Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles.RNA (New York, N.Y.) · 2005Article
- Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation.Genetics · 2005Article
- Mutations in U5 snRNA loop 1 influence the splicing of different genes in vivo.Nucleic acids research · 2002Article
- The ribosomal translocase homologue Snu114p is involved in unwinding U4/U6 RNA during activation of the spliceosome.EMBO reports · 2002Article
- The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p.RNA (New York, N.Y.) · 2001Article
- ATP-dependent interaction of yeast U5 snRNA loop 1 with the 5' splice site.RNA (New York, N.Y.) · 2001Article
- An upstream AG determines whether a downstream AG is selected during catalytic step II of splicing.Molecular and cellular biology · 2001Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present here the first insights into the organization of proteins on the RNA in the U5 snRNP of Saccharomyces cerevisiae. Photo-crosslinking with uniformly labeled U5 RNA in snRNPs reconstituted in vitro revealed five contacting proteins, Prp8p, Snu114p, p30, p16, and p10, contact by the three smaller proteins requiring an intact Sm site. Site-specific crosslinking showed that Snu114p contacts the 5' side of internal loop 1, whereas Prp8p interacts with five different regions of the 5' stem-loop, but not with the Sm site or 3' stem-loop. Both internal loops in the 5' domain are essential for Prp8p to associate with the snRNP, but the conserved loop 1 is not, although this is the region to which Prp8p crosslinks most strongly. The extensive contacts between Prp8p and the 5' stem-loop of U5 RNA support the hypothesis that, in spliceosomes, Prp8p stabilizes loop 1-exon interactions. Moreover, data showing that Prp8p contacts the exons even in the absence of loop 1 indicate that Prp8p may be the principal anchoring factor for exons in the spliceosome. This and the close proximity of the spliceosomal translocase, Snu114p, to U5 loop 1 and Prp8p support and extend the proposal that Snu114p mimics U5 loop 1 during a translocation event in the spliceosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.