ArticleJournal of bacteriology1998
Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule: the rkpK gene encodes a UDP-glucose dehydrogenase.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 90 citations in OpenAlex.
- Genomic insights into 'Candidatus Pseudomonas auctus' JDE115, a soybean endophyte with expanded nutrient acquisition and biocontrol-related gene repertoires.BMC genomics · 2026Article
- Probing the core metabolism ofJournal of bacteriology · 2025Article
- Golden EGG, a simplified Golden Gate cloning system to assemble multiple fragments.Scientific reports · 2024Article
- Rhizobium determinants of rhizosphere persistence and root colonization.The ISME journal · 2024Article
- Varietas Delectat: Exploring Natural Variations in Nitrogen-Fixing Symbiosis Research.Frontiers in plant science · 2022Review
- Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes.International journal of molecular sciences · 2021Review
- Exopolysaccharide Characterization ofFrontiers in plant science · 2021Article
- Structure of the unusualThe Journal of biological chemistry · 2020Article
- Mutation in theGenes · 2018Article
- Article
- Bacterial Molecular Signals in the Sinorhizobium fredii-Soybean Symbiosis.International journal of molecular sciences · 2016Article
- A bifunctional glycosyltransferase from Agrobacterium tumefaciens synthesizes monoglucosyl and glucuronosyl diacylglycerol under phosphate deprivation.The Journal of biological chemistry · 2014Article
- Article
- Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life.The ISME journal · 2012Article
- Role for Rhizobium rhizogenes K84 cell envelope polysaccharides in surface interactions.Applied and environmental microbiology · 2012Article
- Absence of functional TolC protein causes increased stress response gene expression in Sinorhizobium meliloti.BMC microbiology · 2010Article
- Identification of tail genes in the temperate phage 16-3 of Sinorhizobium meliloti 41.Journal of bacteriology · 2010Article
- The rkp-1 cluster is required for secretion of Kdo homopolymeric capsular polysaccharide in Sinorhizobium meliloti strain Rm1021.Journal of bacteriology · 2009Article
- Novel genes related to nodulation, secretion systems, and surface structures revealed by a genome draft of Rhizobium tropici strain PRF 81.Functional & integrative genomics · 2009Article
- Glucomannan-mediated attachment of Rhizobium leguminosarum to pea root hairs is required for competitive nodule infection.Journal of bacteriology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of exopolysaccharide (EPS) was shown to be required for the infection process by rhizobia that induce the formation of indeterminate nodules on the roots of leguminous host plants. In Sinorhizobium meliloti (also known as Rhizobium meliloti) Rm41, a capsular polysaccharide (KPS) analogous to the group II K antigens of Escherichia coli can replace EPS during symbiotic nodule development and serve as an attachment site for the strain-specific bacteriophage phi16-3. The rkpA to -J genes in the chromosomal rkp-1 region code for proteins that are involved in the synthesis, modification, and transfer of an as-yet-unknown lipophilic molecule which might function as a specific lipid carrier during KPS biosynthesis. Here we report that with a phage phi16-3-resistant population obtained after random Tn5 mutagenesis, we have identified novel mutants impaired in KPS production by genetic complementation and biochemical studies. The mutations represent two novel loci, designated the rkp-2 and rkp-3 regions, which are required for the synthesis of rhizobial KPS. The rkp-2 region harbors two open reading frames (ORFs) organized in monocistronic transcription units. Although both genes are required for normal lipopolysaccharide production, only the second one, designated rkpK, is involved in the synthesis of KPS. We have demonstrated that RkpK possesses UDP-glucose dehydrogenase activity, while the protein product of ORF1 might function as a UDP-glucuronic acid epimerase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.