ArticlePlant physiology1998
Domains of a transit sequence required for in vivo import in Arabidopsis chloroplasts.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Turnip mosaic virus utilizes the lipid droplet biogenesis machinery to facilitate its propagation in plants.The New phytologist · 2026Article
- Review
- The ten amino acids of the oxygen-evolving enhancer of tobacco is sufficient as the peptide residues for protein transport to the chloroplast thylakoid.Plant molecular biology · 2021Article
- The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis.Frontiers in plant science · 2021Article
- Article
- Article
- Proteomic Analysis of Lipid Droplets from Arabidopsis Aging Leaves Brings New Insight into Their Biogenesis and Functions.Frontiers in plant science · 2017Article
- Non-native, N-terminal Hsp70 molecular motor recognition elements in transit peptides support plastid protein translocation.The Journal of biological chemistry · 2015Article
- Role of plastoglobules in metabolite repair in the tocopherol redox cycle.Frontiers in plant science · 2014Article
- Arabidopsis BPG2: a phytochrome-regulated gene whose protein product binds to plastid ribosomal RNAs.Planta · 2012Article
- Differential transit peptide recognition during preprotein binding and translocation into flowering plant plastids.The Plant cell · 2012Article
- Amino acid homeostasis modulates salicylic acid-associated redox status and defense responses in Arabidopsis.The Plant cell · 2010Article
- Metabolic and transcriptional responses of glycerolipid pathways to a perturbation of glycerol 3-phosphate metabolism in Arabidopsis.The Journal of biological chemistry · 2010Article
- A toc159 import receptor mutant, defective in hydrolysis of GTP, supports preprotein import into chloroplasts.The Journal of biological chemistry · 2009Article
- Arabidopsis nuclear-encoded plastid transit peptides contain multiple sequence subgroups with distinctive chloroplast-targeting sequence motifs.The Plant cell · 2008Article
- A novel nucleus-encoded chloroplast protein, PIFI, is involved in NAD(P)H dehydrogenase complex-mediated chlororespiratory electron transport in Arabidopsis.Plant physiology · 2007Article
- Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Plastoglobules: a new address for targeting recombinant proteins in the chloroplast.BMC biotechnology · 2007Article
- The plastid protein THYLAKOID FORMATION1 and the plasma membrane G-protein GPA1 interact in a novel sugar-signaling mechanism in Arabidopsis.The Plant cell · 2006Article
- Functional characterization of sequence motifs in the transit peptide of Arabidopsis small subunit of rubisco.Plant physiology · 2006Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear-encoded precursors of chloroplast proteins are synthesized with an amino-terminal cleavable transit sequence, which contains the information for chloroplastic targeting. To determine which regions of the transit sequence are most important for its function, the chloroplast uptake and processing of a full-length ferredoxin precursor and four mutants with deletions in adjacent regions of the transit sequence were analyzed. Arabidopsis was used as an experimental system for both in vitro and in vivo import. The full-length wild-type precursor translocated efficiently into isolated Arabidopsis chloroplasts, and upon expression in transgenic Arabidopsis plants only mature-sized protein was detected, which was localized inside the chloroplast. None of the deletion mutants was imported in vitro. By analyzing transgenic plants, more subtle effects on import were observed. The most N-terminal deletion resulted in a fully defective transit sequence. Two deletions in the middle region of the transit sequence allowed translocation into the chloroplast, although with reduced efficiencies. One deletion in this region strongly reduced mature protein accumulation in older plants. The most C-terminal deletion was translocated but resulted in defective processing. These results allow the dissection of the transit sequence into separate functional regions and give an in vivo basis for a domain-like structure of the ferredoxin transit sequence.
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