ArticlePlant physiology1998
Shifts of intracellular pH distribution as a part of the signal mechanism leading to the elicitation of benzophenanthridine alkaloids . Phytoalexin biosynthesis in cultured cells of eschscholtzia californica
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 89 citations in OpenAlex.
- Higher accumulation of mitragynine in Mitragyna speciosa (kratom) leaves affected by insect attack.PloS one · 2025Article
- Regulating programmed cell death in plant cells: Intracellular acidification plays a pivotal role together with calcium signaling.The Plant cell · 2024Review
- Anthocyanin in the Vacuole of Red Onion Epidermal Cells Quenches Other Fluorescent Molecules.Plants (Basel, Switzerland) · 2019Article
- pH effects on plant calcium fluxes: lessons from acidification-mediated calcium elevation induced by the γ-glutamyl-leucine dipeptide identified from Phytophthora infestans.Scientific reports · 2019Article
- The Vacuolar Proton-Cation Exchanger EcNHX1 Generates pH Signals for the Expression of Secondary Metabolism in Eschscholzia californica.Plant physiology · 2016Article
- Self-incompatibility-induced programmed cell death in field poppy pollen involves dramatic acidification of the incompatible pollen tube cytosol.Plant physiology · 2015Article
- "Self" and "non-self" in the control of phytoalexin biosynthesis: plant phospholipases A2 with alkaloid-specific molecular fingerprints.The Plant cell · 2015Article
- Optimisation approaches for concurrent transmitted light imaging during confocal microscopy.Plant methods · 2015Article
- How plants cope with heavy metals.Botanical studies · 2014Review
- Intracellular pH distribution as a cell health indicator in Saccharomyces cerevisiae.Journal of the Royal Society, Interface · 2011Article
- Structure and mechanism of sanguinarine reductase, an enzyme of alkaloid detoxification.The Journal of biological chemistry · 2010Article
- Cytosolic calcium and pH signaling in plants under salinity stress.Plant signaling & behavior · 2010Review
- Changes in the effective gravitational field strength affect the state of phosphorylation of stress-related proteins in callus cultures of Arabidopsis thaliana.Journal of experimental botany · 2009Article
- The signal molecule lysophosphatidylcholine in Eschscholzia californica is rapidly metabolized by reacylation.Planta · 2008Article
- Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter.The Plant cell · 2007Article
- Article
- The Galpha protein controls a pH-dependent signal path to the induction of phytoalexin biosynthesis in Eschscholzia californica.The Plant cell · 2006Article
- Convergence of calcium signaling pathways of pathogenic elicitors and abscisic acid in Arabidopsis guard cells.Plant physiology · 2002Article
- Elicitor-activated phospholipase A(2) generates lysophosphatidylcholines that mobilize the vacuolar H(+) pool for pH signaling via the activation of Na(+)-dependent proton fluxes.The Plant cell · 2002Article
- Regulation of CDPKs, including identification of PAL kinase, in biotically stressed cells of French bean.Plant molecular biology · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cultured cells of Eschscholtzia californica (Californian poppy) respond to a yeast elicitor preparation or Penicillium cyclopium spores with the production of benzophenanthridine alkaloids, which are potent phytoalexins. Confocal pH mapping with the probe carboxy-seminaphthorhodafluor-1-acetoxymethylester revealed characteristic shifts of the pH distribution in challenged cells: within a few minutes after elicitor contact a transient acidification of cytoplasmic and nuclear areas occurred in parallel with an increase of the vacuolar pH. The change of proton concentration in the vacuole and in the extravacuolar area showed a nearly constant relation, indicating an efflux of vacuolar protons into the cytosol. A 10-min treatment with 2 mM butyric or pivalic acid caused a transient acidification of the cytoplasm comparable to that observed after elicitor contact and also induced alkaloid biosynthesis. Experimental depletion of the vacuolar proton pool reversibly prevented both the elicitor-triggered pH shifts and the induction of alkaloid biosynthesis. pH shifts and induction of alkaloid biosynthesis showed a similar dependence on the elicitor concentration. Net efflux of K+, alkalinization of the outer medium, and browning of the cells were evoked only at higher elicitor concentrations. We suggest that transient acidification of the cytoplasm via efflux of vacuolar protons is both a necessary and sufficient step in the signal path toward biosynthesis of benzophenanthridine alkaloids in Californian poppy cells.
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