ArticleGenes & development1998
Synapse-specific and neuregulin-induced transcription require an ets site that binds GABPalpha/GABPbeta.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 113 citations in OpenAlex.
- The immortality mechanism of TERT promoter mutant cancers is self-reinforcing and reversible.Molecular cell · 2025Article
- Building, Breaking, and Repairing Neuromuscular Synapses.Cold Spring Harbor perspectives in biology · 2024Review
- Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway.Cell & bioscience · 2021Article
- Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle.Frontiers in molecular neuroscience · 2020Article
- The Structure, Function, and Physiology of the Fetal and Adult Acetylcholine Receptor in Muscle.Frontiers in molecular neuroscience · 2020Review
- GA binding protein augments autophagy via transcriptional activation of BECN1-PIK3C3 complex genes.Autophagy · 2014Article
- The role of MuSK in synapse formation and neuromuscular disease.Cold Spring Harbor perspectives in biology · 2013Review
- Bone marrow-derived mesenchymal stem cells up-regulate acetylcholine receptor delta subunit through NRG/ErbB3-mediated mitogen-activated protein kinase pathway.Clinical and translational science · 2012Article
- Chromatin modifications that support acetylcholine receptor gene activation are established during muscle cell determination and differentiation.Molecular biology reports · 2011Article
- GA-binding protein is dispensable for neuromuscular synapse formation and synapse-specific gene expression.Molecular and cellular biology · 2007Article
- Regulation of the intermediate filament protein nestin at rodent neuromuscular junctions by innervation and activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Targeting of the ETS factor GABPalpha disrupts neuromuscular junction synaptic function.Molecular and cellular biology · 2007Article
- Postsynaptic chromatin is under neural control at the neuromuscular junction.The EMBO journal · 2007Article
- Characterization of a negative transcriptional element in the BRCA1 promoter.Breast cancer research : BCR · 2007Article
- Directing RNA interference specifically to differentiated muscle cells.Journal of muscle research and cell motility · 2007Article
- Neuromuscular synapse formation in mice lacking motor neuron- and skeletal muscle-derived Neuregulin-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Identification of a crucial site for synoviolin expression.Molecular and cellular biology · 2005Article
- LL5beta: a regulator of postsynaptic differentiation identified in a screen for synaptically enriched transcripts at the neuromuscular junction.The Journal of cell biology · 2005Article
- Phosphorylation-elicited quaternary changes of GA binding protein in transcriptional activation.Molecular and cellular biology · 2003Article
- A novel pathway for MuSK to induce key genes in neuromuscular synapse formation.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Localization of acetylcholine receptors (AChRs) to neuromuscular synapses is mediated by multiple pathways. Agrin, which is the signal for one pathway, stimulates a redistribution of previously unlocalized AChRs to synaptic sites. The signal for a second pathway is not known, but this signal stimulates selective transcription of AChR genes in myofiber nuclei located near the synaptic site. Neuregulin (NRG) is a good candidate for the extracellular signal that induces synapse-specific gene expression, since NRG is concentrated at synaptic sites and activates AChR gene expression in cultured muscle cells. Previous studies have demonstrated that 181 bp of 5' flanking DNA from the AChR delta-subunit gene are sufficient to confer synapse-specific transcription in transgenic mice and NRG responsiveness in cultured muscle cells, but the critical sequences within this cis-acting regulatory region have not been identified. We transfected AChR delta-subunit-hGH gene fusions into a muscle cell line, and we show that a potential binding site for Ets proteins is required for NRG-induced gene expression. Furthermore, we produced transgenic mice carrying AChR delta-subunit-hGH gene fusions with a mutation in this NRG-response element (NRE), and we show that this NRE is necessary for synapse-specific transcription in mice. The NRE binds proteins in myotube nuclear extracts, and nucleotides that are important for NRG responsiveness are likewise critical for formation of the protein-DNA complex. This complex contains GABPalpha, an Ets protein, and GABPbeta, a protein that lacks an Ets domain but dimerizes with GABPalpha, because formation of the protein-DNA complex is inhibited by antibodies to either GABPalpha or GABPbeta. These results demonstrate that synapse-specific and NRG-induced gene expression require an Ets-binding site and suggest that GABPalpha/GABPbeta mediates the transcriptional response of the AChR delta-subunit gene to synaptic signals, including NRG.
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