ArticleThe Journal of physiology1998
Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- Purinergic signaling in testes revealed.The Journal of general physiology · 2016Article
- Estrogenic regulation of bicarbonate transporters from SLC4 family in rat Sertoli cells.Molecular and cellular biochemistry · 2015Article
- Thyroid hormone and leptin in the testis.Frontiers in endocrinology · 2014Review
- New approaches to thyroid hormones and purinergic signaling.Journal of thyroid research · 2013Article
- Tubular fluid secretion in the seminiferous epithelium: ion transporters and aquaporins in Sertoli cells.The Journal of membrane biology · 2010Review
- The cystic fibrosis transmembrane conductance regulator in reproductive health and disease.The Journal of physiology · 2009Review
- Do seminiferous tubules secrete a fluid rich in KHCO3?The Journal of physiology · 2002Article
- Failure of spermatogenesis in mouse lines deficient in the Na(+)-K(+)-2Cl(-) cotransporter.The Journal of clinical investigation · 2000Article
- Regulated anion secretion in cultured epithelia from Sertoli cells of immature rats.The Journal of physiology · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Cultured epithelia of Sertoli cells from prepubertal rats were grown on Matrigel-coated millipore filters for short-circuit current (Isc) measurements. Under basal conditions, these epithelia exhibited a 'zero' transepithelial potential difference, a 'zero' short-circuit current and a transepithelial resistance of 60 Omega cm2. 2. Forskolin (100 microM) and 8-(4-chlorophenylthio)-cAMP (cpt-cAMP) (100 microM) added to the apical side stimulated the Isc (forskolin, peak DeltaIsc = 1.32 +/- 0.16 microA cm-1; cpt-cAMP, peak DeltaIsc = 0.88 +/- 0.16 microA cm-2). 3. ATP (100 microM) added apically elicited a Isc response (peak DeltaIsc = 6.45 +/- 0. 28 microA cm-2) which was similar in magnitude to that of 1 microM thapsigargin (peak DeltaIsc = 6.09 +/- 0.44 microA cm-2). The potency of the responses to other nucleotides: UTP >= ATP > ADP >> AMP = adenosine indicates the involvement of a mixture of P2Y receptors. 4. Removal of extracellular Cl- and HCO3- reduced the Isc response to ATP by 70 % and 40 %, respectively. Removal of K+ had no effect, whereas removal of Na+ attenuated the Isc response. 5. The response to ATP was insensitive to agents known to block anion secretion (except apical diphenylamine-2-carboxylate (DPC) and DIDS). The resistance to perturbation by pharmacological agents may be a unique property of the seminiferous epithelium. 6. Whole-cell current recordings in cultured rat Sertoli cells demonstrated a DIDS-sensitive outwardly rectifying Cl- conductance with activating and inactivating characteristics at depolarizing and hyperpolarizing voltages, respectively. 7. The stimulation of electrogenic ion transport by ATP may be part of a complex mechanism regulating fluid secretion by the testis. Cultured Sertoli cell epithelia are shown to provide a useful model to investigate transepithelial transport in the seminiferous epithelium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.