ArticleThe Journal of physiology1998
Fibre type-specific gene expression activated by chronic electrical stimulation of adult mouse skeletal muscle fibres in culture.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Alternating bipolar field stimulation identifies muscle fibers with defective excitability but maintained local Ca(2+) signals and contraction.Skeletal muscle · 2016Article
- Both short intense and prolonged moderate in vitro stimulation reduce the mRNA expression of calcium-regulatory proteins in rat skeletal muscle.Molecular and cellular biochemistry · 2013Article
- In situ detection and measurement of intracellular reactive oxygen species in single isolated mature skeletal muscle fibers by real time fluorescence microscopy.Antioxidants & redox signaling · 2008Article
- Activity- and calcineurin-independent nuclear shuttling of NFATc1, but not NFATc3, in adult skeletal muscle fibers.Molecular biology of the cell · 2006Article
- Parallel mechanisms for resting nucleo-cytoplasmic shuttling and activity dependent translocation provide dual control of transcriptional regulators HDAC and NFAT in skeletal muscle fiber type plasticity.Journal of muscle research and cell motility · 2006Review
- Activity-dependent and -independent nuclear fluxes of HDAC4 mediated by different kinases in adult skeletal muscle.The Journal of cell biology · 2005Article
- Signaling pathways in activity-dependent fiber type plasticity in adult skeletal muscle.Journal of muscle research and cell motility · 2005Review
- Fast-to-slow transformation and nuclear import/export kinetics of the transcription factor NFATc1 during electrostimulation of rabbit muscle cells in culture.The Journal of physiology · 2002Article
- Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers.The Journal of cell biology · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Fast-twitch skeletal muscle fibres were enzymatically dissociated from adult mouse flexor digitorum brevis (FDB) muscles and maintained in culture without or with chronic low frequency stimulation (one 5 s train of 5 Hz pulses per minute) for up to 6 days. Single fibre reverse transcriptase-polymerase chain reaction was conducted to coamplify beta-myosin heavy chain (beta-MHC) and alpha-skeletal actin mRNA from the same fibre. 2. Chronic low frequency electrical stimulation of FDB fibres in culture increased the level of mRNA for beta-MHC. In unstimulated fibres there was a slight decline in the beta-MHC mRNA level. As an internal control there was no increase in the level of mRNA for alpha-actin in the identical individual stimulated or unstimulated fibres. 3. Neither the percentage of fibres exhibiting beta-MHC protein nor the Ca2+ transients recorded from individual fibres subjected to the same pattern of stimulation showed any difference between stimulated and unstimulated fibres over the period in culture. 4. This system provides a convenient in vitro model system for studying activity-dependent control of fibre type-specific gene expression in adult skeletal muscle fibres in culture.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.