ArticleMolecular biology of the cell1998
Selection of gbeta subunits with point mutations that fail to activate specific signaling pathways in vivo: dissecting cellular responses mediated by a heterotrimeric G protein in Dictyostelium discoideum.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Function and Regulation of Heterotrimeric G Proteins during Chemotaxis.International journal of molecular sciences · 2016Review
- A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis.Developmental cell · 2012Article
- Electrical signaling in control of ocular cell behaviors.Progress in retinal and eye research · 2012Review
- Cells navigate with a local-excitation, global-inhibition-biased excitable network.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The signaling mechanisms underlying cell polarity and chemotaxis.Cold Spring Harbor perspectives in biology · 2009Review
- Directional sensing in eukaryotic chemotaxis: a balanced inactivation model.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Quantitative imaging of single live cells reveals spatiotemporal dynamics of multistep signaling events of chemoattractant gradient sensing in Dictyostelium.Molecular biology of the cell · 2005Article
- A G alpha-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement.Genes & development · 2004Article
- A Phytophthora infestans G-protein beta subunit is involved in sporangium formation.Eukaryotic cell · 2003Article
- Ggamma in dictyostelium: its role in localization of gbetagamma to the membrane is required for chemotaxis in shallow gradients.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In Dictyostelium discoideum, a unique Gbeta subunit is required for a G protein-coupled receptor system that mediates a variety of cellular responses. Binding of cAMP to cAR1, the receptor linked to the G protein G2, triggers a cascade of responses, including activation of adenylyl cyclase, gene induction, actin polymerization, and chemotaxis. Null mutations of the cAR1, Galpha2, and Gbeta genes completely impair all these responses. To dissect specificity in Gbetagamma signaling to downstream effectors in living cells, we screened a randomly mutagenized library of Gbeta genes and isolated Gbeta alleles that lacked the capacity to activate some effectors but retained the ability to regulate others. These mutant Gbeta subunits were able to link cAR1 to G2, to support gene expression, and to mediate cAMP-induced actin polymerization, and some were able to mediate to chemotaxis toward cAMP. None was able to activate adenylyl cyclase, and some did not support chemotaxis. Thus, we separated in vivo functions of Gbetagamma by making point mutations on Gbeta. Using the structure of the heterotrimeric G protein displayed in the computer program CHAIN, we examined the positions and the molecular interactions of the amino acids substituted in each of the mutant Gbetas and analyzed the possible effects of each replacement. We identified several residues that are crucial for activation of the adenylyl cyclase. These residues formed an area that overlaps but is not identical to regions where bovine Gtbetagamma interacts with its regulators, Galpha and phosducin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.