ArticleMolecular biology of the cell1998
The dynamic behavior of individual microtubules associated with chromosomes in vitro.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Higher-order protein assembly controls kinetochore formation.Nature cell biology · 2024Article
- Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity.Nature communications · 2019Article
- In vitro reconstitution of lateral to end-on conversion of kinetochore-microtubule attachments.Methods in cell biology · 2018Article
- Review series: The functions and consequences of force at kinetochores.The Journal of cell biology · 2013Review
- Spindle pole mechanics studied in mitotic asters: dynamic distribution of spindle forces through compliant linkages.Biophysical journal · 2011Article
- "Artificial mitotic spindle" generated by dielectrophoresis and protein micropatterning supports bidirectional transport of kinesin-coated beads.Integrative biology : quantitative biosciences from nano to macro · 2011Article
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Force transduction by the microtubule-bound Dam1 ring.Biophysical journal · 2010Article
- In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces.Methods in cell biology · 2010Review
- Nonequilibrium self-assembly of a filament coupled to ATP/GTP hydrolysis.Biophysical journal · 2009Article
- Microtubule assembly dynamics at the nanoscale.Current biology : CB · 2007Article
- A driving and coupling "Pac-Man" mechanism for chromosome poleward translocation in anaphase A.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The bacterial actin-like cytoskeleton.Microbiology and molecular biology reviews : MMBR · 2006Review
- Microtubule movements on the arms of mitotic chromosomes: polar ejection forces quantified in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Microtubule self-organisation by reaction-diffusion processes causes collective transport and organisation of cellular particles.BMC cell biology · 2004Article
- Yeast kinetochores do not stabilize Stu2p-dependent spindle microtubule dynamics.Molecular biology of the cell · 2003Article
- Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle.Biophysical journal · 2003Article
- A simple, mechanistic model for directional instability during mitotic chromosome movements.Biophysical journal · 2002Article
- Activation of the MKK/ERK pathway during somatic cell mitosis: direct interactions of active ERK with kinetochores and regulation of the mitotic 3F3/2 phosphoantigen.The Journal of cell biology · 1998Article
- Microtubules move the nucleus to quiescence.Nucleus (Austin, Tex.)Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Mitotic movements of chromosomes are usually coupled to the elongation and shortening of the microtubules to which they are bound. The lengths of kinetochore-associated microtubules change by incorporation or loss of tubulin subunits, principally at their chromosome-bound ends. We have reproduced aspects of this phenomenon in vitro, using a real-time assay that displays directly the movements of individual chromosome-associated microtubules as they elongate and shorten. Chromosomes isolated from cultured Chinese hamster ovary cells were adhered to coverslips and then allowed to bind labeled microtubules. In the presence of tubulin and GTP, these microtubules could grow at their chromosome-bound ends, causing the labeled segments to move away from the chromosomes, even in the absence of ATP. Sometimes a microtubule would switch to shortening, causing the direction of movement to change abruptly. The link between a microtubule and a chromosome was mechanically strong; 15 pN of tension was generally insufficient to detach a microtubule, even though it could add subunits at the kinetochore-microtubule junction. The behavior of the microtubules in vitro was regulated by the chromosomes to which they were bound; the frequency of transitions from polymerization to depolymerization was decreased, and the speed of depolymerization-coupled movement toward chromosomes was only one-fifth the rate of shortening for microtubules free in solution. Our results are consistent with a model in which each microtubule interacts with an increasing number of chromosome-associated binding sites as it approaches the kinetochore.
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