ArticleMolecular biology of the cell1998
In vitro reconstitution of microtubule plus end-directed, GTPgammaS-sensitive motility of Golgi membranes.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Golgi isolation.Cold Spring Harbor protocols · 2015Article
- Cdc42 regulates microtubule-dependent Golgi positioning.Traffic (Copenhagen, Denmark) · 2010Article
- DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis.Plant physiology · 2005Article
- Microtubule-dependent movement of late endocytic vesicles in vitro: requirements for Dynein and Kinesin.Molecular biology of the cell · 2004Article
- Tension in tubulovesicular networks of Golgi and endoplasmic reticulum membranes.Biophysical journal · 2004Article
- The EF-hand Ca2+-binding protein p22 plays a role in microtubule and endoplasmic reticulum organization and dynamics with distinct Ca2+-binding requirements.Molecular biology of the cell · 2004Article
- Microtubule and motor-dependent endocytic vesicle sorting in vitro.The Journal of cell biology · 2000Article
- In vitro formation of the endoplasmic reticulum occurs independently of microtubules by a controlled fusion reaction.The Journal of cell biology · 2000Article
- Brefeldin A-dependent membrane tubule formation reconstituted in vitro is driven by a cell cycle-regulated microtubule motor.Molecular biology of the cell · 2000Article
- Allele-specific activators and inhibitors for kinesin.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Microtubule-based endoplasmic reticulum motility in Xenopus laevis: activation of membrane-associated kinesin during development.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Purified Golgi membranes were mixed with cytosol and microtubules (MTs) and observed by video enhanced light microscopy. Initially, the membranes appeared as vesicles that moved along MTs. As time progressed, vesicles formed aggregates from which membrane tubules emerged, traveled along MTs, and eventually generated extensive reticular networks. Membrane motility required ATP, occurred mainly toward MT plus ends, and was inhibited almost completely by the H1 monoclonal antibody to kinesin heavy chain, 5'-adenylylimidodiphosphate, and 100 microM but not 20 microM vanadate. Motility was also blocked by GTPgammaS or A1F4- but was insensitive to A1C13, NaF, staurosporin, or okadaic acid. The targets for GTPgammaS and A1F4- were evidently of cytosolic origin, did not include kinesin or MTs, and were insensitive to several probes for trimeric G proteins. Transport of Golgi membranes along MTs mediated by a kinesin has thus been reconstituted in vitro. The motility is regulated by one or more cytosolic GTPases but not by protein kinases or phosphatases that are inhibited by staurosporin or okadaic acid, respectively. The pertinent GTPases are likely to be small G proteins or possibly dynamin. The in vitro motility may correspond to Golgi-to-ER or Golgi-to-cell surface transport in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.