ArticleThe Journal of cell biology1998
Cre-loxP-mediated inactivation of the alpha6A integrin splice variant in vivo: evidence for a specific functional role of alpha6A in lymphocyte migration but not in heart development.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- The Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors.Communications biology · 2022Article
- α6-Integrin alternative splicing: distinct cytoplasmic variants in stem cell fate specification and niche interaction.Stem cell research & therapy · 2018Review
- Control of Hoxd gene transcription in the mammary bud by hijacking a preexisting regulatory landscape.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Regulated splicing of the α6 integrin cytoplasmic domain determines the fate of breast cancer stem cells.Cell reports · 2014Article
- Lack of exon 10 in the murine tau gene results in mild sensorimotor defects with aging.BMC neuroscience · 2013Article
- Transgene- and locus-dependent imprinting reveals allele-specific chromosome conformations.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Impact of copy number variations (CNVs) on long-range gene regulation at the HoxD locus.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Integrin α6β4 in colorectal cancer.World journal of gastrointestinal pathophysiology · 2010Article
- Article
- Endothelial basement membrane laminin alpha5 selectively inhibits T lymphocyte extravasation into the brain.Nature medicine · 2009Article
- Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The impact of alternative splicing in vivo: mouse models show the way.RNA (New York, N.Y.) · 2007Review
- Review
- Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line.Immunology · 2004Article
- Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan.The Journal of cell biology · 2003Article
- Extension and integration of the gene ontology (GO): combining GO vocabularies with external vocabularies.Genome research · 2002Article
- Function of the tetraspanin CD151-alpha6beta1 integrin complex during cellular morphogenesis.Molecular biology of the cell · 2002Article
- Phosphorylation of a conserved integrin alpha 3 QPSXXE motif regulates signaling, motility, and cytoskeletal engagement.Molecular biology of the cell · 2001Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two splice variants of the alpha6 integrin subunit, alpha6A and alpha6B, with different cytoplasmic domains, have previously been described. While alpha6B is expressed throughout the development of the mouse, the expression of alpha6A begins at 8.5 days post coitum and is initially restricted to the myocardium. Later in ontogeny, alpha6A is found in various epithelia and in certain cells of the immune system. In this study, we have investigated the function of alpha6A in vivo by generating knockout mice deficient for this splice variant. The Cre- loxP system of the bacteriophage P1 was used to specifically remove the exon encoding the cytoplasmic domain of alpha6A in embryonic stem cells, and the deletion resulted in the expression of alpha6B in all tissues that normally express alpha6A. We show that alpha6A-/- mice develop normally and are fertile. The substitution of alpha6A by alpha6B does not impair the development and function of the heart, hemidesmosome formation in the epidermis, or keratinocyte migration. Furthermore, T cells differentiated normally in alpha6A-/- mice. However, the substitution of alpha6A by alpha6B leads to a decrease in the migration of lymphocytes through laminin-coated Transwell filters and to a reduction of the number of T cells isolated from the peripheral and mesenteric lymph nodes. Lymphocyte homing to the lymph nodes, which involves various types of integrin-ligand interactions, was not affected in the alpha6A knockout mice, indicating that the reduced number of lymph node cells could not be directly attributed to defects in lymphocyte trafficking. Nevertheless, the expression of alpha6A might be necessary for optimal lymphocyte migration on laminin in certain pathological conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.