ArticleThe Journal of cell biology1998
Clb5-associated kinase activity is required early in the spindle pathway for correct preanaphase nuclear positioning in Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Measuring Regulatory Network Inheritance in Dividing Yeast Cells Using Ordinary Differential Equations.bioRxiv : the preprint server for biology · 2025Article
- Unveiling Forkhead-mediated regulation of yeast cell cycle and metabolic networks.Computational and structural biotechnology journal · 2022Article
- Cyclin/Forkhead-mediated coordination of cyclin waves: an autonomous oscillator rationalizing the quantitative model of Cdk control for budding yeast.NPJ systems biology and applications · 2021Review
- Article
- Quantitative analysis of triple-mutant genetic interactions.Nature protocols · 2014Article
- Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation.Cell reports · 2013Article
- Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae.Eukaryotic cell · 2011Article
- Inhibition of epithelial-to-mesenchimal transition: a novel tumor suppressor function of p53?Cell cycle (Georgetown, Tex.) · 2011Article
- TORC1 kinase and the S-phase cyclin Clb5 collaborate to promote mitotic spindle assembly and DNA replication in S. cerevisiae.Current genetics · 2010Article
- Specific genetic interactions between spindle assembly checkpoint proteins and B-Type cyclins in Saccharomyces cerevisiae.Genetics · 2009Article
- The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast.Molecular biology of the cell · 2007Article
- Yeast UBL-UBA proteins have partially redundant functions in cell cycle control.Cell division · 2006Article
- The CLIP-170 homologue Bik1p promotes the phosphorylation and asymmetric localization of Kar9p.Molecular biology of the cell · 2006Article
- Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex.Genes & development · 2004Article
- The Arabidopsis locus RCB mediates upstream regulation of mitotic gene expression.Plant physiology · 2003Article
- Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions.The EMBO journal · 2003Article
- Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae.Molecular biology of the cell · 2002Article
- Article
- Article
- Dynamic localization of the Swe1 regulator Hsl7 during the Saccharomyces cerevisiae cell cycle.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, a single cyclin-dependent kinase, Cdc28, regulates both G1/S and G2/M phase transitions by associating with stage-specific cyclins. During progression through S phase and G2/M, Cdc28 is activated by the B-type cyclins Clb1-6. Because of functional redundancy, specific roles for individual Clbs have been difficult to assign. To help genetically define such roles, strains carrying a cdc28(ts) allele, combined with single CLB deletions were studied. We assumed that by limiting the activity of the kinase, these strains would be rendered more sensitive to loss of individual Clbs. By this approach, a novel phenotype associated with CLB5 mutation was observed. Homozygous cdc28-4(ts) clb5 diploids were inviable at room temperature. Cells were defective in spindle positioning, leading to migration of undivided nuclei into the bud. Occasionally, misplaced spindles were observed in cdc28-4 clb5 haploids; additional deletion of CLB6 caused full penetrance. Thus, CLB5 effects proper preanaphase spindle positioning, yet the requirement differs in haploids and diploids. The execution point for the defect corresponded to the time of Clb5-dependent kinase activation. Nevertheless, lethality of cdc28-4 clb5 diploids was not rescued by CLB2 or CLB4 overexpression, indicating a specificity of Clb5 function beyond temporality of expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.