ArticleThe Journal of cell biology1998
Why are two different cross-linkers necessary for actin bundle formation in vivo and what does each cross-link contribute?
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.The Journal of cell biology · 2026Article
- Role of BicDR in bristle shaft construction and support of BicD functions.Journal of cell science · 2024Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Lactate-mediated Fascin protrusions promote cell adhesion and migration in cervical cancer.Theranostics · 2023Article
- Scale-type-specific requirement for the mosquito Aedes aegypti Spindle-F homologue by regulating microtubule organization.Insect molecular biology · 2022Article
- TheFrontiers in cellular and infection microbiology · 2022Article
- Recapitulating Actin Module Organization in theInternational journal of molecular sciences · 2021Article
- How does fascin promote cancer metastasis?The FEBS journal · 2021Review
- A binding protein regulates myosin-7a dimerization and actin bundle assembly.Nature communications · 2021Article
- Model for Bundling of Keratin Intermediate Filaments.Biophysical journal · 2020Article
- Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments.Cell reports · 2019Article
- The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.iScience · 2019Article
- Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons.PLoS biology · 2019Article
- Structural Insights into the Induced-fit Inhibition of Fascin by a Small-Molecule Inhibitor.Journal of molecular biology · 2018Article
- Fascin- and α-Actinin-Bundled Networks Contain Intrinsic Structural Features that Drive Protein Sorting.Current biology : CB · 2016Article
- IKKε inhibits PKC to promote Fascin-dependent actin bundling.Development (Cambridge, England) · 2016Article
- Improving fascin inhibitors to block tumor cell migration and metastasis.Molecular oncology · 2016Article
- Fascin Rigidity and L-plastin Flexibility Cooperate in Cancer Cell Invadopodia and Filopodia.The Journal of biological chemistry · 2016Article
- Targeted inhibition of fascin function blocks tumour invasion and metastatic colonization.Nature communications · 2015Article
- Inner ear tissue preservation by rapid freezing: improving fixation by high-pressure freezing and hybrid methods.Hearing research · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In developing Drosophila bristles two species of cross-linker, the forked proteins and fascin, connect adjacent actin filaments into bundles. Bundles form in three phases: (a) tiny bundles appear; (b) these bundles aggregate into larger bundles; and (c) the filaments become maximally cross-linked by fascin. In mutants that completely lack forked, aggregation of the bundles does not occur so that the mature bundles consist of <50 filaments versus approximately 700 for wild type. If the forked concentration is genetically reduced to half the wild type, aggregation of the tiny bundles occurs but the filaments are poorly ordered albeit with small patches of fascin cross-linked filaments. In mutants containing an excess of forked, all the bundles tend to aggregate and the filaments are maximally crossbridged by fascin. Alternatively, if fascin is absent, phases 1 and 2 occur normally but the resultant bundles are twisted and the filaments within them are poorly ordered. By extracting fully elongated bristles with potassium iodide which removes fascin but leaves forked, the bundles change from being straight to twisted and the filaments within them become poorly ordered. From these observations we conclude that (a) forked is used early in development to aggregate the tiny bundles into larger bundles; and (b) forked facilitates fascin entry into the bundles to maximally cross-link the actin filaments into straight, compact, rigid bundles. Thus, forked aligns the filaments and then directs fascin binding so that inappropriate cross-linking does not occur.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.