ArticleThe Journal of cell biology1998
Sorting mechanisms regulating membrane protein traffic in the apical transcytotic pathway of polarized MDCK cells.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 98 citations in OpenAlex.
- The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- The WDR11 complex facilitates the tethering of AP-1-derived vesicles.Nature communications · 2018Article
- Membrane Transport across Polarized Epithelia.Cold Spring Harbor perspectives in biology · 2017Review
- CLIC4 regulates apical exocytosis and renal tube luminogenesis through retromer- and actin-mediated endocytic trafficking.Nature communications · 2016Article
- Simplifying the synthesis of SIgA: combination of dIgA and rhSC using affinity chromatography.Methods (San Diego, Calif.) · 2014Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells.Developmental cell · 2012Article
- Article
- Apical protein transport and lumen morphogenesis in polarized epithelial cells.Bioscience reports · 2011Review
- Article
- The role of secretory antibodies in infection immunity.Nature reviews. Microbiology · 2010Review
- Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation.The Journal of biological chemistry · 2010Article
- Cell models for studying renal physiology.Pflugers Archiv : European journal of physiology · 2008Review
- Differential involvement of endocytic compartments in the biosynthetic traffic of apical proteins.The EMBO journal · 2007Article
- Receptor complexes cotransported via polarized endocytic pathways form clusters with distinct organizations.Molecular biology of the cell · 2007Article
- Cholesterol-sensitive modulation of transcytosis.Molecular biology of the cell · 2007Article
- Clathrin and clathrin-accessory proteins in rat kidney cortex epithelia.Histochemistry and cell biology · 2006Article
- Differential trafficking of transforming growth factor-beta receptors and ligand in polarized epithelial cells.Molecular biology of the cell · 2004Article
- Endosomal ricin transport: involvement of Rab4- and Rab5-positive compartments.Histochemistry and cell biology · 2004Article
- Bidirectional transepithelial IgG transport by a strongly polarized basolateral membrane Fcgamma-receptor.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
The transcytotic pathway followed by the polymeric IgA receptor (pIgR) carrying its bound ligand (dIgA) from the basolateral to the apical surface of polarized MDCK cells has been mapped using morphological tracers. At 20 degreesC dIgA-pIgR internalize to interconnected groups of vacuoles and tubules that comprise the endosomal compartment and in which they codistribute with internalized transferrin receptors (TR) and epidermal growth factor receptors (EGFR). Upon transfer to 37 degreesC the endosome vacuoles develop long tubules that give rise to a distinctive population of 100-nm-diam cup-shaped vesicles containing pIgR. At the same time, the endosome gives rise to multivesicular endosomes (MVB) enriched in EGFR and to 60-nm-diam basolateral vesicles. The cup-shaped vesicles carry the dIgA/pIgR complexes to the apical surface where they exocytose. Using video microscopy and correlative electron microscopy to study cells grown thin and flat we show that endosome vacuoles tubulate in response to dIgA/pIgR but that the tubules contain TR as well as pIgR. However, we show that TR are removed from these dIgA-induced tubules via clathrin-coated buds and, as a result, the cup-shaped vesicles to which the tubules give rise become enriched in dIgA/pIgR. Taken together with the published information available on pIgR trafficking signals, our observations suggest that the steady-state concentrations of TR and unoccupied pIgR on the basolateral surface of polarized MDCK cells are maintained by a signal-dependent, clathrin-based sorting mechanism that operates along the length of the transcytotic pathway. We propose that the differential sorting of occupied receptors within the MDCK endosome is achieved by this clathrin-based mechanism continuously retrieving receptors like TR from the pathways that deliver pIgR to the apical surface and EGFR to the lysosome.
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