ArticleThe Plant cell1998
Inefficient reinitiation is responsible for upstream open reading frame-mediated translational repression of the maize R gene.
Article in The Plant cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 76 citations in OpenAlex.
- Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Ribo-Seq Analysis-Based Elucidation of the Dynamic Translation Landscape of Yak Ovarian Tissues in Different Reproductive Stages.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Anthocyanin metabolism in Nelumbo: translational and post-translational regulation control transcription.BMC plant biology · 2023Article
- uORFlight: a vehicle toward uORF-mediated translational regulation mechanisms in eukaryotes.Database : the journal of biological databases and curation · 2020Article
- Transcriptome and Proteome Profiling of Different Colored Rice Reveals Physiological Dynamics Involved in the Flavonoid Pathway.International journal of molecular sciences · 2019Article
- Complex regulation of the TaMyc1 gene expression in wheat grain synthesizing anthocyanin pigments.Molecular biology reports · 2018Article
- A uORF Represses the Transcription Factor AtHB1 in Aerial Tissues to Avoid a Deleterious Phenotype.Plant physiology · 2017Article
- Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2016Article
- Combining in silico prediction and ribosome profiling in a genome-wide search for novel putatively coding sORFs.BMC genomics · 2013Article
- Translational Regulation of Cytoplasmic mRNAs.The arabidopsis book · 2013Article
- Genome-wide identification and characterization of R2R3MYB family in Cucumis sativus.PloS one · 2012Article
- Unified translation repression mechanism for microRNAs and upstream AUGs.BMC genomics · 2010Article
- Alternative splicing of anciently exonized 5S rRNA regulates plant transcription factor TFIIIA.Genome research · 2009Article
- Conserved upstream open reading frames in higher plants.BMC genomics · 2008Article
- On the functions of the h subunit of eukaryotic initiation factor 3 in late stages of translation initiation.Genome biology · 2007Article
- "HAIRY CANOLA"--Arabidopsis GL3 induces a dense covering of trichomes on Brassica napus seedlings.Plant molecular biology · 2006Article
- The Arabidopsis STV1 protein, responsible for translation reinitiation, is required for auxin-mediated gynoecium patterning.The Plant cell · 2005Article
- Frequency and character of alternative somatic recombination fates of paralogous genes during T-DNA integration.Molecular genetics and genomics : MGG · 2005Article
- Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h.The Plant cell · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize R genes encode a small family of transcriptional activators of several structural genes in the anthocyanin biosynthetic pathway. The 5' leader region of most R genes contains a 38-codon upstream open reading frame (uORF) that previously was shown to be responsible for the repression of downstream gene expression in a transient transformation assay. In this study, we report that the 5' leader also can repress translation of the downstream luciferase gene both in the rabbit reticulocyte translation system and in transgenic rice plants. The ability to visualize the uORF peptide after in vitro translation permits quantification of both products of dicistronic mRNAs. Similarly, the construction of transgenic rice plants expressing wild-type and mutant constructs permits the quantification and correlation of steady state mRNA levels and reporter gene activities. Using these assays, we demonstrate directly that translation of the uORF is required for repression, that increasing translation of the uORF peptide decreases downstream gene expression, and that repression is unaffected by either subtle or gross changes in the uORF peptide. Rather, we find that ribosomes that translate the uORF reinitiate inefficiently and that the intercistronic sequence downstream of the uORF mediates this effect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.