ArticleThe Journal of general physiology1998
Permeation and gating of an inwardly rectifying potassium channel. Evidence for a variable energy well.
Article in The Journal of general physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Mutation in pore-helix modulates interplay between filter gate and Ba2+ block in a Kcv channel pore.The Journal of general physiology · 2024Article
- Vascular inward rectifier K+ channels as external K+ sensors in the control of cerebral blood flow.Microcirculation (New York, N.Y. : 1994) · 2015Review
- A comprehensive guide to the ROMK potassium channel: form and function in health and disease.American journal of physiology. Renal physiology · 2009Review
- Serotonin excites fast-spiking interneurons in the striatum.The European journal of neuroscience · 2009Article
- Role of conserved glycines in pH gating of Kir1.1 (ROMK).Biophysical journal · 2006Article
- A ring of negative charges in the intracellular vestibule of Kir2.1 channel modulates K+ permeation.Biophysical journal · 2005Article
- Molecular diversity and regulation of renal potassium channels.Physiological reviews · 2005Review
- Carboxy-terminal determinants of conductance in inward-rectifier K channels.The Journal of general physiology · 2004Article
- Evolving potassium channels by means of yeast selection reveals structural elements important for selectivity.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The pore helix is involved in stabilizing the open state of inwardly rectifying K+ channels.Biophysical journal · 2003Article
- Influence of permeant ions on gating in cyclic nucleotide-gated channels.The Journal of general physiology · 2003Article
- IRK1 inward rectifier K(+) channels exhibit no intrinsic rectification.The Journal of general physiology · 2002Article
- Permeant ion-dependent changes in gating of Kir2.1 inward rectifier potassium channels.The Journal of general physiology · 2001Article
- Mutations within the P-loop of Kir6.2 modulate the intraburst kinetics of the ATP-sensitive potassium channel.The Journal of general physiology · 2001Article
- Ammonium ions induce inactivation of Kir2.1 potassium channels expressed in Xenopus oocytes.The Journal of physiology · 2001Article
- Expression and permeation properties of the K(+) channel Kir7.1 in the retinal pigment epithelium.The Journal of physiology · 2001Article
- The K+ channel signature sequence of murine Kir2.1: mutations that affect microscopic gating but not ionic selectivity.The Journal of physiology · 2001Article
- Permeation properties of inward-rectifier potassium channels and their molecular determinants.The Journal of general physiology · 2000Article
- A mutation linked with Bartter's syndrome locks Kir 1.1a (ROMK1) channels in a closed state.The Journal of general physiology · 1999Article
- Structural determinants of gating in inward-rectifier K+ channels.Biophysical journal · 1999Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Permeation, gating, and their interrelationship in an inwardly rectifying potassium (K+) channel, ROMK2, were studied using heterologous expression in Xenopus oocytes. Patch-clamp recordings of single channels were obtained in the cell-attached mode. The gating kinetics of ROMK2 were well described by a model having one open and two closed states. One closed state was short lived (approximately 1 ms) and the other was longer lived (approximately 40 ms) and less frequent (approximately 1%). The long closed state was abolished by EDTA, suggesting that it was due to block by divalent cations. These closures exhibit a biphasic voltage dependence, implying that the divalent blockers can permeate the channel. The short closures had a similar biphasic voltage dependence, suggesting that they could be due to block by monovalent, permeating cations. The rate of entering the short closed state varied with the K+ concentration and was proportional to current amplitude, suggesting that permeating K+ ions may be related to the short closures. To explain the results, we propose a variable intrapore energy well model in which a shallow well may change into a deep one, resulting in a normally permeant K+ ion becoming a blocker of its own channel.
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