Evidence map›Paper›PMID 9758849›Full record

ArticleApplied and environmental microbiology1998

Effective recovery of bacterial DNA and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens.

J H Apajalahti, L K Särkilahti, B R Mäki, J P Heikkinen, P H Nurminen, W E Holben

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 211 citations in OpenAlex.

  1. Trial
  2. Can probiotics improve the environmental microbiome and resistome of commercial poultry production?International journal of environmental research and public health · 2013
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  18. Effects of dietary fiber levels on cecal microbiota composition in geese.Asian-Australasian journal of animal sciences · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

J H ApajalahtiCultor Corporation Technology Center, FIN-02460, Kantvik, Finland. juha.apajalahti@cultor.com
L K Särkilahti
B R Mäki
J P Heikkinen
P H Nurminen
W E Holben
University of Montana · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A DNA-based, direct method for initial characterization of the total bacterial community in ileum and cecum of the chicken gastrointestinal (GI) tract was developed. The efficiencies of bacterial extraction and lysis were >95 and >99%, respectively, and therefore the DNA recovered should accurately reflect the bacterial communities of the ileal and cecal digesta. Total bacterial DNA samples were fractionated according to their percent G+C content. The profiles reflecting the composition of the bacterial community were reproducible within each compartment, but different between the compartments of the GI tract. This approach is independent of the culturability of the bacteria in the consortium and can be used to improve our understanding of how diet and other variables modulate the microbial communities of the GI tracts of animals.

Indexed as

Animal FeedAnimalsBacteriaBase CompositionCecumChickensCytosineDigestive SystemDNA, BacterialGastrointestinal ContentsGuanineIleumReproducibility of ResultsCytosineDNA, BacterialGuanine

Identifiers

PMID9758849
PMCPMC106608
OpenAlexW2105046957

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.