ArticleApplied and environmental microbiology1998
A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 104 citations in OpenAlex.
- Natural variation of the wheat root exudate metabolome and its influence on biological nitrification inhibition activity.Plant biotechnology journal · 2025Article
- Phenylpropanoid methyl esterase unlocks catabolism of aromatic biological nitrification inhibitors.The ISME journal · 2025Article
- Assessing the activity of different plant-derived molecules and potential biological nitrification inhibitors on a range of soil ammonia- and nitrite-oxidizing strains.Applied and environmental microbiology · 2023Article
- Root exudate fingerprint ofFrontiers in molecular biosciences · 2023Article
- Maize-Brachiaria intercropping: A strategy to supply recycled N to maize and reduce soil NAgriculture, ecosystems & environment · 2021Article
- The Sorghum bicolor Root Exudate Sorgoleone Shapes Bacterial Communities and Delays Network Formation.mSystems · 2021Article
- Biological Nitrification Inhibition (BNI): Phenotyping of a Core Germplasm Collection of the Tropical Forage GrassFrontiers in plant science · 2020Article
- Evaluation of dimethyl sulfoxide (DMSO) as a co-solvent for toxicity testing of hydrophobic organic compounds.Ecotoxicology (London, England) · 2019Article
- Pesticide Side Effects in an Agricultural Soil Ecosystem as Measured by amoA Expression Quantification and Bacterial Diversity Changes.PloS one · 2015Article
- A paradigm shift towards low-nitrifying production systems: the role of biological nitrification inhibition (BNI).Annals of botany · 2013Review
- A potentiometric flow biosensor based on ammonia-oxidizing bacteria for the detection of toxicity in water.Sensors (Basel, Switzerland) · 2013Article
- Stimulatory effect of xenobiotics on oxidative electron transport of chemolithotrophic nitrifying bacteria used as biosensing element.PloS one · 2013Article
- Article
- Selective enhancement of the fluorescent pseudomonad population after amending the recirculating nutrient solution of hydroponically grown plants with a nitrogen stabilizer.Microbial ecology · 2008Article
- Solid-phase contact assay that uses a lux-marked Nitrosomonas europaea reporter strain to estimate toxicity of bioavailable linear alkylbenzene sulfonate in soil.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An expression vector for the luxAB genes, derived from Vibrio harveyi, was introduced into Nitrosomonas europaea. Although the recombinant strain produced bioluminescence due to the expression of the luxAB genes under normal growing conditions, the intensity of the light emission decreased immediately, in a time-and dose-dependent manner, with the addition of ammonia monooxygenase inhibitors, such as allylthiourea, phenol, and nitrapyrin. When whole cells were challenged with several nitrification inhibitors and toxic compounds, a close relationship was found between the change in the intensity of the light emission and the level of ammonia-oxidizing activity. The response of bioluminescence to the addition of allylthiourea was considerably faster than the change in the ammonia-oxidizing rate, measured as both the O2 uptake and NO2- production rates. The bioluminescence of cells inactivated by ammonia monooxygenase inhibitor was recovered rapidly by the addition of certain substrates for hydroxylamine oxidoreductase. These results suggested that the inhibition of bioluminescence was caused by the immediate decrease of reducing power in the cell due to the inactivation of ammonia monooxygenase, as well as by the destruction of other cellular metabolic pathways. We conclude that the assay system using luminous Nitrosomonas can be applied as a rapid and sensitive detection test for nitrification inhibitors, and it will be used to monitor the nitrification process in wastewater treatment plants.
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