ArticleGenetics1998
An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.The Plant cell · 2020Article
- Evidence for ESCRT- and clathrin-dependent microautophagy.The Journal of cell biology · 2017Article
- GTPase cross talk regulates TRAPPII activation of Rab11 homologues during vesicle biogenesis.The Journal of cell biology · 2016Article
- The endosomal protein-sorting receptor sortilin has a role in trafficking α-1 antitrypsin.Genetics · 2012Article
- Phospholipid flippases: building asymmetric membranes and transport vesicles.Biochimica et biophysica acta · 2012Review
- Vesicle trafficking from a lipid perspective: Lipid regulation of exocytosis in Saccharomyces cerevisiae.Cellular logistics · 2012Article
- Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.PloS one · 2012Article
- The antibiotic gentamicin inhibits specific protein trafficking functions of the Arf1/2 family of GTPases.Antimicrobial agents and chemotherapy · 2011Article
- Article
- The antidepressant sertraline targets intracellular vesiculogenic membranes in yeast.Genetics · 2010Article
- Linking phospholipid flippases to vesicle-mediated protein transport.Biochimica et biophysica acta · 2009Review
- Clathrin functions in the absence of the terminal domain binding site for adaptor-associated clathrin-box motifs.Molecular biology of the cell · 2009Article
- Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue.Molecular biology of the cell · 2009Article
- Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Genome-scale gene/reaction essentiality and synthetic lethality analysis.Molecular systems biology · 2009Article
- Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway.Molecular biology of the cell · 2007Article
- Dissection of Swa2p/auxilin domain requirements for cochaperoning Hsp70 clathrin-uncoating activity in vivo.Molecular biology of the cell · 2006Article
- Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi.The EMBO journal · 2006Article
- Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Vps10p cycles between the TGN and the late endosome via the plasma membrane in clathrin mutants.Molecular biology of the cell · 2002Article
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2 authors.
Funding
Abstract
ADP-ribosylation factor (ARF) is a small GTP-binding protein that is thought to regulate the assembly of coat proteins on transport vesicles. To identify factors that functionally interact with ARF, we have performed a genetic screen in Saccharomyces cerevisiae for mutations that exhibit synthetic lethality with an arf1Delta allele and defined seven genes by complementation tests (SWA1-7 for synthetically lethal with arf1Delta). Most of the swa mutants exhibit phenotypes comparable to arf1Delta mutants such as temperature-conditional growth, hypersensitivity to fluoride ions, and partial protein transport and glycosylation defects. Here, we report that swa5-1 is a new temperature-sensitive allele of the clathrin heavy chain gene (chc1-5), which carries a frameshift mutation near the 3' end of the CHC1 open reading frame. This genetic interaction between arf1 and chc1 provides in vivo evidence for a role for ARF in clathrin coat assembly. Surprisingly, strains harboring chc1-5 exhibited a significant defect in transport of carboxypeptidase Y or carboxypeptidase S to the vacuole that was not observed in other chc1 ts mutants. The kinetics of invertase secretion or transport of alkaline phosphatase to the vacuole were not significantly affected in the chc1-5 mutant, further implicating clathrin specifically in the Golgi to vacuole transport pathway for carboxypeptidase Y.
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