ArticleThe EMBO journal1998
sigmaR, an RNA polymerase sigma factor that modulates expression of the thioredoxin system in response to oxidative stress in Streptomyces coelicolor A3(2).
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 198 citations in OpenAlex.
- Hypoxia-induced production of the cyclolipodepsipeptide BE-43547 byMicrobiology (Reading, England) · 2026Article
- Sigma Factor Engineering inMicroorganisms · 2024Article
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances inInternational journal of molecular sciences · 2022Article
- RIViT-seq enables systematic identification of regulons of transcriptional machineries.Nature communications · 2022Article
- Overlapping SigH and SigE sigma factor regulons inFrontiers in microbiology · 2022Article
- PG1659 functions as anti-sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83.Molecular oral microbiology · 2021Article
- A Proteomic Analysis Indicates That Oxidative Stress Is the Common Feature Triggering Antibiotic Production inFrontiers in microbiology · 2021Article
- The Alternative Sigma Factor RpoE2 Is Involved in the Stress Response to Hypochlorite andFrontiers in microbiology · 2021Article
- Rewiring the specificity of extracytoplasmic function sigma factors.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Expression of genes of the Pho regulon is altered in Streptomyces coelicolor.Scientific reports · 2020Article
- Discovery of the extracytoplasmic function σ factors.Molecular microbiology · 2019Review
- The genome sequence of Streptomyces rochei 7434AN4, which carries a linear chromosome and three characteristic linear plasmids.Scientific reports · 2019Article
- Complex general stress response regulation in Sphingomonas melonis Fr1 revealed by transcriptional analyses.Scientific reports · 2019Article
- The actinobacterial WhiB-like (Wbl) family of transcription factors.Molecular microbiology · 2018Review
- Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis.ACS infectious diseases · 2018Article
- Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters.Microbial cell factories · 2018Article
- Characterization of Sigma Factor Genes inFrontiers in microbiology · 2018Article
- Article
- Monitoring global protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis under hypochlorite stress.Scientific reports · 2017Article
- Themes and variations in gene regulation by extracytoplasmic function (ECF) sigma factors.Current opinion in microbiology · 2017Review
24 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified an RNA polymerase sigma factor, sigmaR, that is part of a system that senses and responds to thiol oxidation in the Gram-positive, antibiotic-producing bacterium Streptomyces coelicolor A3(2). Deletion of the gene (sigR) encoding sigmaR caused sensitivity to the thiol-specific oxidant diamide and to the redox cycling compounds menadione and plumbagin. This correlated with reduced levels of disulfide reductase activity and an inability to induce this activity on exposure to diamide. The trxBA operon, encoding thioredoxin reductase and thioredoxin, was found to be under the direct control of sigmaR. trxBA is transcribed from two promoters, trxBp1 and trxBp2, separated by 5-6 bp. trxBp1 is transiently induced at least 50-fold in response to diamide treatment in a sigR-dependent manner. Purified sigmaR directed transcription from trxBp1 in vitro, indicating that trxBp1 is a target for sigmaR. Transcription of sigR itself initiates at two promoters, sigRp1 and sigRp2, which are separated by 173 bp. The sigRp2 transcript was undetectable in a sigR-null mutant, and purified sigmaR could direct transcription from sigRp2 in vitro, indicating that sigR is positively autoregulated. Transcription from sigRp2 was also transiently induced (70-fold) following treatment with diamide. We propose a model in which sigmaR induces expression of the thioredoxin system in response to cytoplasmic disulfide bond formation. Upon reestablishment of normal thiol levels, sigmaR activity is switched off, resulting in down-regulation of trxBA and sigR. We present evidence that the sigmaR system also functions in the actinomycete pathogen Mycobacterium tuberculosis.
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