ArticleThe EMBO journal1998
Importance of a flexible hinge near the motor domain in kinesin-driven motility.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Toxoplasma Gondii Importin α Shows Weak Auto-Inhibition.The protein journal · 2023Article
- Structural Correlation of the Neck Coil with the Coiled-coil (CC1)-Forkhead-associated (FHA) Tandem for Active Kinesin-3 KIF13A.The Journal of biological chemistry · 2016Article
- Dynamic microtubules drive circuit rewiring in the absence of neurite remodeling.Current biology : CB · 2015Article
- Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development.Gene expression patterns : GEP · 2013Article
- Secondary structure and compliance of a predicted flexible domain in kinesin-1 necessary for cooperation of motors.Biophysical journal · 2008Article
- Processive kinesins require loose mechanical coupling for efficient collective motility.EMBO reports · 2008Article
- The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Review: regulation mechanisms of Kinesin-1.Journal of muscle research and cell motility · 2006Review
- The E-hook of tubulin interacts with kinesin's head to increase processivity and speed.Biophysical journal · 2005Article
- Investigating the putative glycine hinge in Shaker potassium channel.The Journal of general physiology · 2005Article
- The complex interplay between the neck and hinge domains in kinesin-1 dimerization and motor activity.Molecular biology of the cell · 2005Article
- Breathing down the neck of Unc104.The Journal of cell biology · 2003Review
- Distinct conformations of the kinesin Unc104 neck regulate a monomer to dimer motor transition.The Journal of cell biology · 2003Article
- Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.The Journal of cell biology · 2003Article
- Single fungal kinesin motor molecules move processively along microtubules.Biophysical journal · 2003Article
- A conserved tyrosine in the neck of a fungal kinesin regulates the catalytic motor core.The EMBO journal · 2003Article
- Hedgehog signal transduction proteins: contacts of the Fused kinase and Ci transcription factor with the kinesin-related protein Costal2.BMC developmental biology · 2002Article
- Unusual properties of the fungal conventional kinesin neck domain from Neurospora crassa.The EMBO journal · 2001Article
- Kinetic equilibrium of forces and molecular events in muscle contraction.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Lethal kinesin mutations reveal amino acids important for ATPase activation and structural coupling.The Journal of biological chemistry · 1999Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional kinesin is a molecular motor consisting of an N-terminal catalytic motor domain, an extended stalk and a small globular C-terminus. Whereas the structure and function of the catalytic motor domain has been investigated, little is known about the function of domains outside the globular head. A short coiled-coil region adjacent to the motor domain, termed the neck, is known to be important for dimerization and may be required for kinesin processivity. We now provide evidence that a helix-disrupting hinge region (hinge 1) that separates the neck from the first extended coiled-coil of the stalk plays an essential role in basic motor activity. A fast fungal kinesin from Syncephalastrum racemosum was used for these studies. Deletion, substitution by a coiled-coil and truncation of the hinge 1 region all reduce motor speed and uncouple ATP turnover from gliding velocity. Insertion of hinge 1 regions from two conventional kinesins, Nkin and DmKHC, fully restores motor activity, whereas insertion of putative flexible linkers of other proteins does not, suggesting that hinge 1 regions of conventional kinesins can functionally replace each other. We suggest that this region is essential for kinesin movement in its promotion of chemo-mechanical coupling of the two heads and therefore the functional motor domain should be redefined to include not only the catalytic head but also the adjacent neck and hinge 1 domains.
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