ArticleProceedings of the National Academy of Sciences of the United States of America1998
The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 288 citations in OpenAlex.
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- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- Spraying Zinc Sulfate to Reveal the Mechanism through the Glutathione Metabolic Pathway Regulates the Cadmium Tolerance of Seashore Paspalum (Plants (Basel, Switzerland) · 2023Article
- Cadmium Transport in Maize Root Segments Using a Classical Physiological Approach: Evidence of Influx Largely Exceeding Efflux in Subapical Regions.Plants (Basel, Switzerland) · 2023Article
- Cadmium alters whole animal ionome and promotes the re-distribution of iron in intestinal cells ofFrontiers in physiology · 2023Article
- Rice Plants (BioMed research international · 2022Article
- Integrative application of heavy metal-resistant bacteria, moringa extracts, and nano-silicon improves spinach yield and declines its contaminant contents on a heavy metal-contaminated soil.Frontiers in plant science · 2022Article
- Advances in molecular mechanisms underlying cadmium uptake and translocation in rice.Frontiers in plant science · 2022Review
- Long-term acclimation to cadmium exposure reveals extensive phenotypic plasticity in Chlamydomonas.Plant physiology · 2021Article
- Overexpression of OsLCT2, a Low-Affinity Cation Transporter Gene, Reduces Cadmium Accumulation in Shoots and Grains of Rice.Rice (New York, N.Y.) · 2021Article
- WDR5a functions in cadmium-inhibited root meristem growth by regulating nitric oxide accumulation in Arabidopsis.Planta · 2020Article
- Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation inInternational journal of molecular sciences · 2020Article
- Cadmium Uptake by Wheat (Plants (Basel, Switzerland) · 2020Review
- Plant Membrane Transport Research in the Post-genomic Era.Plant communications · 2020Review
- Polyamines modulate growth, antioxidant activity, and genomic DNA in heavy metal-stressed wheat plant.Environmental science and pollution research international · 2019Article
- Salicylic Acid Signals Plant Defence against Cadmium Toxicity.International journal of molecular sciences · 2019Review
- Plant Salinity Stress: Many Unanswered Questions Remain.Frontiers in plant science · 2019Review
- The pathway of transmembrane cadmium influx via calcium-permeable channels and its spatial characteristics along rice root.Journal of experimental botany · 2018Article
- Article
- Silicon deposition in roots minimizes the cadmium accumulation and oxidative stress in leaves of cowpea plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nonessential metal ions such as cadmium are most likely transported across plant membranes via transporters for essential cations. To identify possible pathways for Cd2+ transport we tested putative plant cation transporters for Cd2+ uptake activity by expressing cDNAs in Saccharomyces cerevisiae and found that expression of one clone, LCT1, renders the growth of yeast more sensitive to cadmium. Ion flux assays showed that Cd2+ sensitivity is correlated with an increase in Cd2+ uptake. LCT1-dependent Cd2+ uptake is saturable, lies in the high-affinity range (apparent KM for Cd2+ = 33 microM) and is sensitive to block by La3+ and Ca2+. Growth assays demonstrated a sensitivity of LCT1-expressing yeast cells to extracellular millimolar Ca2+ concentrations. LCT1-dependent increase in Ca2+ uptake correlated with the observed phenotype. Furthermore, LCT1 complements a yeast disruption mutant in the MID1 gene, a non-LCT1-homologous yeast gene encoding a membrane Ca2+ influx system required for recovery from the mating response. We conclude that LCT1 mediates the uptake of Ca2+ and Cd2+ in yeast and may therefore represent a first plant cDNA encoding a plant Ca2+ uptake or an organellar Ca2+ transport pathway in plants and may contribute to transport of the toxic metal Cd2+ across plant membranes.
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