ArticleProceedings of the National Academy of Sciences of the United States of America1998
Trans-chromosomal recombination within the Ig heavy chain switch region in B lymphocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Loop extrusion promotes an alternate pathway for isotype switching.Cell reports · 2021Article
- IgH 3' regulatory region increases ectopic class switch recombination.PLoS genetics · 2021Article
- Long-Range Control of Class Switch Recombination by Transcriptional Regulatory Elements.Frontiers in immunology · 2021Review
- Double-stranded DNA break polarity skews repair pathway choice during intrachromosomal and interchromosomal recombination.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- RNA Exosome and Non-coding RNA-Coupled Mechanisms in AID-Mediated Genomic Alterations.Journal of molecular biology · 2017Review
- The IgH locus 3' cis-regulatory super-enhancer co-opts AID for allelic transvection.Oncotarget · 2017Article
- Article
- Origin of immunoglobulin isotype switching.Current biology : CB · 2012Article
- Gene Conversion-Like Events in the Diversification of Human Rearranged IGHV3-23*01 Gene Sequences.Frontiers in immunology · 2012Article
- Reassessment of the role of Mut S homolog 5 in Ig class switch recombination shows lack of involvement in cis- and trans-switching.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- AID to overcome the limitations of genomic information by introducing somatic DNA alterations.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2006Review
- Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene.Genome research · 2000Article
- Article
- Immunoglobulin genes undergo legitimate repair in human B cells not only after cis- but also frequent trans-class switch recombination.Genes and immunityArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Somatic DNA rearrangements in B lymphocytes, including V(D)J gene rearrangements and isotype switching, generally occur in cis, i. e., intrachromosomally. We showed previously, however, that 3 to 7% of IgA heavy chains have the VH and Calpha regions encoded in trans. To determine whether the trans-association of VH and Calpha occurred by trans-chromosomal recombination, by trans-splicing, or by trans-chromosomal gene conversion, we generated and analyzed eight IgA-secreting rabbit hybridomas with trans-associated VH and Calpha heavy chains. By ELISA and by nucleotide sequence analysis we found that the VH and Calpha regions were encoded by genes that were in trans in the germline. We cloned the rearranged VDJ-Calpha gene from a fosmid library of one hybridoma and found that the expressed VH and Calpha genes were juxtaposed. Moreover, the juxtaposed VH and Calpha genes originated from different IgH alleles. From the same hybridoma, we also identified a fosmid clone with the other expected product of a trans-chromosomal recombination. The recombination breakpoint occurred within the Smicro/Salpha region, indicating that the trans-association of VH and Calpha genes occurred by trans-chromosomal recombination during isotype switching. We conclude that trans-chromosomal recombination occurs at an unexpectedly high frequency (7%) within the IgH locus of B lymphocytes in normal animals, which may explain the high incidence of B-cell tumors that arise from oncogene translocation into the IgH locus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.