ArticleProceedings of the National Academy of Sciences of the United States of America1998
Promoter opening via a DNA fork junction binding activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 90 citations in OpenAlex.
- Single-molecule characterization of extrinsic transcription termination by Sen1 helicase.Nature communications · 2019Article
- Single-molecule analysis reveals the mechanism of transcription activation inScience advances · 2018Article
- Modulation of extracytoplasmic function (ECF) sigma factor promoter selectivity by spacer region sequence.Nucleic acids research · 2018Article
- Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation.Molecular cell · 2017Article
- The bacterial enhancer-dependent RNA polymerase.The Biochemical journal · 2016Review
- RNA polymerase molecular beacon as tool for studies of RNA polymerase-promoter interactions.Methods (San Diego, Calif.) · 2015Review
- TRANSCRIPTION. Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies.Science (New York, N.Y.) · 2015Article
- Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Crystal structures of the E. coli transcription initiation complexes with a complete bubble.Molecular cell · 2015Article
- Base flipping in open complex formation at bacterial promoters.Biomolecules · 2015Review
- Coupling of downstream RNA polymerase-promoter interactions with formation of catalytically competent transcription initiation complex.Journal of molecular biology · 2014Article
- Insights into the mechanism of initial transcription in Escherichia coli RNA polymerase.The Journal of biological chemistry · 2013Article
- Cooperativity and interaction energy threshold effects in recognition of the -10 promoter element by bacterial RNA polymerase.Nucleic acids research · 2013Article
- Structure and function of CarD, an essential mycobacterial transcription factor.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- RNA polymerase-promoter interactions determining different stability of the Escherichia coli and Thermus aquaticus transcription initiation complexes.Nucleic acids research · 2012Article
- The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.Microbiology and molecular biology reviews : MMBR · 2012Review
- Article
- Structural basis for promoter-10 element recognition by the bacterial RNA polymerase σ subunit.Cell · 2011Article
- Molecular mechanism of transcription inhibition by phage T7 gp2 protein.Journal of molecular biology · 2011Article
- Promoter spacer DNA plays an active role in integrating the functional consequences of RNA polymerase contacts with -10 and -35 promoter elements.Biophysical chemistry · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rate-limiting step in transcriptional initiation typically is opening the promoter DNA to expose the template strand. Opening is tightly regulated, but how it occurs is not known. These experiments identify an activity, recognition of specific DNA fork junctions, and suggest that it is critical to bacterial promoter opening. This activity is both sequence and structure specific; it recognizes the bases that constitute the upstream double-stranded/single-stranded boundary of the open complex. Promoter mutations known to reduce opening rates lead to comparable reductions in fork junction binding affinity. The activity acts to establish the upstream boundary of melted DNA and works in conjunction with two single-stranded DNA binding activities that recognize separately the two melted strands. The junction binding activity is contained within the sigma factor component of the holoenzyme. The activity occurs in both a typical prokaryotic transcription system and in a eukaryotic-like bacterial system that responds to enhancers and needs ATP. Thus DNA opening catalyzed by fork junction binding may occur in a variety of systems in which DNA must be opened to be copied.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.