ArticleJournal of bacteriology1998
Carbon-source-dependent expression of the PalkB promoter from the Pseudomonas oleovorans alkane degradation pathway.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 99 citations in OpenAlex.
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- Metabolic and Evolutionary Insights in the Transformation of Diphenylamine by aFrontiers in microbiology · 2018Article
- The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows Tightly Controlled Gene Expression over a Wide Range in Pseudomonas aeruginosa.Applied and environmental microbiology · 2016Article
- The Interaction between Plants and Bacteria in the Remediation of Petroleum Hydrocarbons: An Environmental Perspective.Frontiers in microbiology · 2016Review
- Marine hydrocarbonoclastic bacteria as whole-cell biosensors for n-alkanes.Microbial biotechnology · 2015Article
- Salix purpurea Stimulates the Expression of Specific Bacterial Xenobiotic Degradation Genes in a Soil Contaminated with Hydrocarbons.PloS one · 2015Article
- Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains.Frontiers in microbiology · 2015Article
- Analyses of both the alkB gene transcriptional start site and alkB promoter-inducing properties of Rhodococcus sp. strain BCP1 grown on n-alkanes.Applied and environmental microbiology · 2011Article
- PcaO positively regulates pcaHG of the beta-ketoadipate pathway in Corynebacterium glutamicum.Journal of bacteriology · 2010Article
- Product repression of alkane monooxygenase expression in Pseudomonas butanovora.Journal of bacteriology · 2006Article
- Levels and activity of the Pseudomonas putida global regulatory protein Crc vary according to growth conditions.Journal of bacteriology · 2005Article
- Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes.Nature biotechnology · 2005Article
- Specificity at the end of the tunnel: understanding substrate length discrimination by the AlkB alkane hydroxylase.Journal of bacteriology · 2005Article
- Regulation of tetralin biodegradation and identification of genes essential for expression of thn operons.Journal of bacteriology · 2004Article
- The Pseudomonas putida Crc global regulator controls the expression of genes from several chromosomal catabolic pathways for aromatic compounds.Journal of bacteriology · 2004Article
- Measurement of biologically available naphthalene in gas and aqueous phases by use of a Pseudomonas putida biosensor.Applied and environmental microbiology · 2004Article
- Expression of the Pseudomonas putida OCT plasmid alkane degradation pathway is modulated by two different global control signals: evidence from continuous cultures.Journal of bacteriology · 2003Article
- Differential expression of the components of the two alkane hydroxylases from Pseudomonas aeruginosa.Journal of bacteriology · 2003Article
- Inactivation of cytochrome o ubiquinol oxidase relieves catabolic repression of the Pseudomonas putida GPo1 alkane degradation pathway.Journal of bacteriology · 2002Article
- Characterization and application of xylene monooxygenase for multistep biocatalysis.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas oleovorans GPo1 can metabolize medium-chain-length alkanes by means of an enzymatic system whose induction is regulated by the AlkS protein. In the presence of alkanes, AlkS activates the expression of promoter PalkB, from which most of the genes of the pathway are transcribed. In addition, expression of the first enzyme of the pathway, alkane hydroxylase, is known to be influenced by the carbon source present in the growth medium, indicating the existence of an additional overimposed level of regulation associating expression of the alk genes with the metabolic status of the cell. Reporter strains bearing PalkB-lacZ transcriptional fusions were constructed to analyze the influence of the carbon source on induction of the PalkB promoter by a nonmetabolizable inducer. Expression was most efficient when cells grew at the expense of citrate, decreasing significantly when the carbon source was lactate or succinate. When cells were grown in Luria-Bertani rich medium, PalkB was strongly down-regulated. This effect was partially relieved when multiple copies of the gene coding for the AlkS activator were present and was not observed when the promoter was moved to Escherichia coli, a heterologous genetic background. Possible mechanisms responsible for PalkB regulation are discussed.
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