ArticlePlant molecular biology1998
Apple ACC-oxidase and polygalacturonase: ripening-specific gene expression and promoter analysis in transgenic tomato.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 97 citations in OpenAlex.
- Fruit-Specific Promoters in Plants: Advances, Regulatory Mechanisms and Applications in Plant Biotechnology.Plants (Basel, Switzerland) · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Olive Oil-Based Lipid Coating as a Precursor Organogel for Postharvest Preservation of Lychee: Efficacy Combined with Polyamide/Polyethylene Packaging Under Passive Atmosphere.Gels (Basel, Switzerland) · 2025Article
- Identification and application of an exocarp-preferential promoter for genetic engineering of tomato fruit.Horticulture research · 2024Article
- A long noncoding RNA functions in high-light-induced anthocyanin accumulation in apple by activating ethylene synthesis.Plant physiology · 2022Article
- Genome-Wide Identification and Functional Investigation ofPlants (Basel, Switzerland) · 2021Article
- Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology.BMC biotechnology · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Fast and Furious: Ethylene-Triggered Changes in the Metabolism of Papaya Fruit During Ripening.Frontiers in plant science · 2019Review
- The Peach RGF/GLV Signaling Peptide pCTG134 Is Involved in a Regulatory Circuit That Sustains Auxin and Ethylene Actions.Frontiers in plant science · 2017Article
- The Grapevine VvPMEI1 Gene Encodes a Novel Functional Pectin Methylesterase Inhibitor Associated to Grape Berry Development.PloS one · 2015Article
- Post-harvest quality risks by stress/ethylene: management to mitigate.Protoplasma · 2015Review
- Genetic and environmental control of fruit maturation, dry matter and firmness in apple (Malus × domestica Borkh.).Horticulture research · 2014Article
- Temporal and spatial control of gene expression in horticultural crops.Horticulture research · 2014Review
- Subchromoplast sequestration of carotenoids affects regulatory mechanisms in tomato lines expressing different carotenoid gene combinations.The Plant cell · 2013Article
- Molecular and genetic regulation of fruit ripening.Plant molecular biology · 2013Review
- Down-regulation of POLYGALACTURONASE1 alters firmness, tensile strength and water loss in apple (Malus x domestica) fruit.BMC plant biology · 2012Article
- A functional pectin methylesterase inhibitor protein (SolyPMEI) is expressed during tomato fruit ripening and interacts with PME-1.Plant molecular biology · 2012Article
- Isolation of a citrus promoter specific for reproductive organs and its functional analysis in isolated juice sacs and tomato.Plant cell reports · 2011Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Levels of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and polygalacturonase (PG) mRNAs were characterized during ripening of Royal Gala, Braeburn and Granny Smith apples. Both ACC-oxidase and PG mRNAs were up-regulated in ripening fruit of all three cultivars. Expression in Royal Gala was detected earlier than in Braeburn and Granny Smith, relative to internal ethylene concentration. Genomic clones corresponding to the ACC-oxidase and PG mRNAs expressed in ripe apple fruit were isolated and ca. 2 kb of each promoter was sequenced. The start point of transcription in each gene was mapped by primer extension, and sequences homologous to elements in other ethylene-responsive or PG promoters were identified. The fruit specificity of the apple ACC-oxidase and PG promoters was investigated in transgenic tomato plants using a nested set of promoter fragments fused to the beta-glucuronidase (gusA) reporter gene. For the ACC-oxidase gene, 450 bp of 5' promoter sequence was sufficient to drive GUS expression, although this expression was not specific to ripening fruit. Larger fragments of 1966 and 1159 bp showed both fruit and ripening specificity. For the PG gene, promoter fragments of 1460 and 532 bp conferred ripening-specific expression in transgenic tomato fruit. However GUS expression was down-regulated by 2356 bp of promoter, suggesting the presence of a negative regulatory element between positions -1460 and -2356.
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