ArticlePlant molecular biology1998
Cre/lox-mediated site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana by transient expression of cre.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 86 citations in OpenAlex.
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Synthetic memory circuits for stable cell reprogramming in plants.Nature biotechnology · 2022Article
- Targeted DNA insertion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.Plant biotechnology journal · 2019Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion.BMC biotechnology · 2015Article
- The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.BMC biotechnology · 2012Article
- Site-specific transgenesis in Xenopus.Genesis (New York, N.Y. : 2000) · 2012Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Ehrlichia chaffeensis tandem repeat proteins and Ank200 are type 1 secretion system substrates related to the repeats-in-toxin exoprotein family.Frontiers in cellular and infection microbiology · 2011Article
- Towards identifying genes underlying ecologically relevant traits in Arabidopsis thaliana.Nature reviews. Genetics · 2010Review
- PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome.BMC biotechnology · 2010Article
- Overexpression of several Arabidopsis histone genes increases agrobacterium-mediated transformation and transgene expression in plants.The Plant cell · 2009Article
- Expression of a transgene exchanged by the recombinase-mediated cassette exchange (RMCE) method in plants.Plant cell reports · 2009Article
- Marker-free site-specific integration plants.Transgenic research · 2008Article
- Markerless multiple-gene-deletion system for Streptococcus mutans.Applied and environmental microbiology · 2008Article
- Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana.Transgenic research · 2008Article
- Stable recombinase-mediated cassette exchange in Arabidopsis using Agrobacterium tumefaciens.Plant physiology · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cre/lox system was used to obtain targeted integration of an Agrobacterium T-DNA at a lox site in the genome of Arabidopsis thaliana. Site-specific recombinants, and not random events, were preferentially selected by activation of a silent lox-neomycin phosphotransferase (nptII) target gene. To analyse the effectiveness of Agrobacterium-mediated transfer we used T-DNA vectors harbouring a single lox sequence (this vector had to circularize at the T-DNA left- and right-border sequences prior to site-specific integration) or two lox sequences (this vector allowed circularization at the lox sequences within the T-DNA either prior to or after random integration, followed by targeting of the circularized vector), respectively. Furthermore, to control the reversibility of the integration reaction, Cre recombinase was provided transiently by using a cotransformation approach. One precise stable integrant was found amongst the recombinant calli obtained after transformation with a double-lox T-DNA vector. The results indicate that Agrobacterium-mediated transformation can be used as a tool to obtain site-specific integration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.