ArticleMedical & biological engineering & computing1998
Regulation of neurofilament interactions in vitro by natural and synthetic polypeptides sharing Lys-Ser-Pro sequences with the heavy neurofilament subunit NF-H: neurofilament crossbridging by antiparallel sidearm overlapping.
Article in Medical & biological engineering & computing, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 61 citations in OpenAlex.
- Dissecting neurofilament tail sequence-phosphorylation-structure relationships with multicomponent reconstituted protein brushes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.Biology open · 2017Article
- Phosphorylation-Induced Mechanical Regulation of Intrinsically Disordered Neurofilament Proteins.Biophysical journal · 2017Article
- Mechanisms and Consequences of Dopamine Depletion-Induced Attenuation of the Spinophilin/Neurofilament Medium Interaction.Neural plasticity · 2017Article
- Mechanical Properties of Intermediate Filament Proteins.Methods in enzymology · 2016Article
- Polyelectrolyte properties of filamentous biopolymers and their consequences in biological fluids.Soft matter · 2014Review
- Conformational properties of interacting neurofilaments: Monte Carlo simulations of cylindrically grafted apposing neurofilament brushes.Journal of biological physics · 2013Article
- Neurofilaments at a glance.Journal of cell science · 2012Review
- How the projection domains of NF-L and alpha-internexin determine the conformations of NF-M and NF-H in neurofilaments.European biophysics journal : EBJ · 2010Article
- Gel-expanded to gel-condensed transition in neurofilament networks revealed by direct force measurements.Nature materials · 2010Article
- Microtubule-independent regulation of neurofilament interactions in vitro by neurofilament-bound ATPase activities.Biochemical and biophysical research communications · 2009Article
- Mechanical and structural properties of in vitro neurofilament hydrogels.European biophysics journal : EBJ · 2007Article
- Softness, strength and self-repair in intermediate filament networks.Experimental cell research · 2007Review
- Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments.Experimental cell research · 2007Review
- Mechanisms of mitochondria-neurofilament interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Relating interactions between neurofilaments to the structure of axonal neurofilament distributions through polymer brush models.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurofilaments are organised into parallel bundles in axons through crossbridges formed by lateral projections of neurofilament subunits. Pure neurofilaments form gels in vitro, consisting of interconnected parallel arrays of filaments regulated by the phosphorylation level of neurofilament subunits. Neurofilament-associated polypeptides sharing phosphorylated epitopes with the repetitive lysine-serine-proline (Lys-Ser-Pro) motifs of the neurofilament heavy subunit sidearm are characterised: they regulate in vitro the neurofilament gelation kinetics in a concentration- and phosphorylation-dependent manner. Studies with synthetic peptides show that interactions between neurofilaments involve both acid and base amino acid residues of neurofilament sidearms and demonstrate the opposite effects of peptides containing either one (inhibition) or two (activation) Lys-Ser-Pro motifs. Electron microscopy reveals an organised network of native neurofilament sidearms, regulated by the phosphorylation level of neurofilament subunits, suggesting a structural transition between intra- and inter-neurofilament sidearm interactions. These results favour the hypothesis of a mechanism of neurofilament crossbridging through the variable antiparallel overlapping of the phosphorylable Lys-Ser-Pro domains of neurofilament sidearms from adjacent filaments, following an equilibrium regulated by neurofilament-associated proteins, bivalent cations and the phosphorylation level of Lys-Ser-Pro motifs from both neurofilament sidearms and neurofilament-associated proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.