ArticleInfection and immunity1998
Identification of two Shigella flexneri chromosomal loci involved in intercellular spreading.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
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- MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis.Scientific reports · 2017Article
- How Do the Virulence Factors ofFrontiers in cellular and infection microbiology · 2017Review
- The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications.Scientific reports · 2016Article
- Molecular and Cellular Mechanisms of Shigella flexneri Dissemination.Frontiers in cellular and infection microbiology · 2016Review
- NtrBC and Nac contribute to efficient Shigella flexneri intracellular replication.Journal of bacteriology · 2014Article
- The Vps/VacJ ABC transporter is required for intercellular spread of Shigella flexneri.Infection and immunity · 2014Article
- The role of oxyR and soxRS in oxidative stress survival in Shigella flexneri.Microbiological research · 2012Article
- Perturbations in histidine biosynthesis uncover robustness in the metabolic network of Salmonella enterica.PloS one · 2012Article
- Genome-scale approaches to identify genes essential for Haemophilus influenzae pathogenesis.Frontiers in cellular and infection microbiology · 2012Review
- The influence of riboflavin and nicotinic acid on Shigella sonnei colony conversion.Iranian journal of microbiology · 2011Article
- CsrA and Cra influence Shigella flexneri pathogenesis.Infection and immunity · 2010Article
- Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability of Shigella flexneri to multiply within colonic epithelial cells and spread to adjacent cells is essential for production of dysentery. Two S. flexneri chromosomal loci that are required for these processes were identified by screening a pool of TnphoA insertion mutants. These mutants were able to invade cultured epithelial cells but could not form wild-type plaques. Analysis of the nucleotide sequence indicated that the sites of TnphoA insertion were within two different regions that are almost identical to Escherichia coli K-12 chromosomal sequences of unknown functions. One region is located at 70 min on the E. coli chromosome, upstream of murZ, while the other is at 28 min, downstream of tonB. The mutant with the insertion at 70 min was named vpsC because it showed an altered pattern of virulence protein secretion. The vpsC mutant formed pinpoint-sized plaques, was defective in recovery from infected tissue culture cells, and was sensitive to lysis by the detergent sodium dodecyl sulfate. Recombinant plasmids carrying the S. flexneri vpsA, -B, and -C genes complemented all of the phenotypes of the vpsC mutant. A mutation in vpsA resulted in the same phenotype as the vpsC mutation, suggesting that these two genes are part of a virulence operon in S. flexneri. The mutant with the insertion at 28 min was interrupted in the same open reading frame as S. flexneri ispA. This ispA mutant could not form plaques and was defective in bacterial septation inside tissue culture cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.