ArticleBiophysical journal1998
Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 169 citations in OpenAlex.
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- G-actin diffusion is insufficient to achieve F-actin assembly in fast-treadmilling protrusions.Biophysical journal · 2023Article
- Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding.Biomolecules · 2023Article
- Cell reorientation on a cyclically strained substrate.PNAS nexus · 2022Article
- A statistical mechanics model for determining the length distribution of actin filaments under cellular tensional homeostasis.Scientific reports · 2022Article
- Balance of osmotic pressures determines the nuclear-to-cytoplasmic volume ratio of the cell.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Rapid ensemble measurement of protein diffusion and probe blinking dynamics in cells.Biophysical reports · 2021Article
- Targeting the cytoskeleton against metastatic dissemination.Cancer metastasis reviews · 2021Review
- Following the footprints of variability during filopodial growth.European biophysics journal : EBJ · 2020Article
- The Arp2/3 complex and the formin, Diaphanous, are both required to regulate the size of germline ring canals in the developing egg chamber.Developmental biology · 2020Article
- Quantitative high-precision imaging of myosin-dependent filamentous actin dynamics.Journal of muscle research and cell motility · 2020Review
- Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth.eLife · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Pulling-force generation by ensembles of polymerizing actin filaments.Physical biology · 2019Article
- Convection-Induced Biased Distribution of Actin Probes in Live Cells.Biophysical journal · 2019Article
- Processive flow by biased polymerization mediates the slow axonal transport of actin.The Journal of cell biology · 2019Article
- Reconsidering an active role for G-actin in cytoskeletal regulation.Journal of cell science · 2018Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The analogous techniques of photoactivation of fluorescence (PAF) and fluorescence recovery after photobleaching (FRAP) have been applied previously to the study of actin dynamics in living cells. Traditionally, separate experiments estimate the mobility of actin monomer or the lifetime of actin filaments. A mathematical description of the dynamics of the actin cytoskeleton, however, predicts that the evolution of fluorescence in PAF and FRAP experiments depends simultaneously on the diffusion coefficient of actin monomer, D, the fraction of actin in filaments, FF, and the lifetime of actin filaments, tau (, Biophys. J. 69:1674-1682). Here we report the application of this mathematical model to the interpretation of PAF and FRAP experiments in subconfluent bovine aortic endothelial cells (BAECs). The following parameters apply for actin in the bulk cytoskeleton of subconfluent BAECs. PAF: D = 3.1 +/- 0.4 x 10(-8) cm2/s, FF = 0.36 +/- 0.04, tau = 7.5 +/- 2.0 min. FRAP: D = 5.8 +/- 1.2 x 10(-8) cm2/s, FF = 0.5 +/- 0.04, tau = 4.8 +/- 0.97 min. Differences in the parameters are attributed to differences in the actin derivatives employed in the two studies and not to inherent differences in the PAF and FRAP techniques. Control experiments confirm the modeling assumption that the evolution of fluorescence is dominated by the diffusion of actin monomer, and the cyclic turnover of actin filaments, but not by filament diffusion. The work establishes the dynamic state of actin in subconfluent endothelial cells and provides an improved framework for future applications of PAF and FRAP.
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