ArticleBiophysical journal1998
MgADP promotes a catch-like state developed through force-calcium hysteresis in tonic smooth muscle.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Creatine kinase, energy reserve, and hypertension: from bench to bedside.Annals of translational medicine · 2018Review
- Phosphate and ADP differently inhibit coordinated smooth muscle myosin groups.Biophysical journal · 2015Article
- Smooth muscle-protein translocation and tissue function.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- Functional characterization of human myosin-18A and its interaction with F-actin and GOLPH3.The Journal of biological chemistry · 2013Article
- SMB myosin heavy chain knockout enhances tonic contraction and reduces the rate of force generation in ileum and stomach antrum.American journal of physiology. Cell physiology · 2013Article
- Tonic and phasic smooth muscle contraction is not regulated by the PKCα - CPI-17 pathway in swine stomach antrum and fundus.PloS one · 2013Article
- Nonmuscle myosin is regulated during smooth muscle contraction.American journal of physiology. Heart and circulatory physiology · 2009Article
- Molecular basis of the catch state in molluscan smooth muscles: a catchy challenge.Journal of muscle research and cell motility · 2008Review
- Regulation of the smooth muscle contractile phenotype by nonmuscle myosin.Journal of muscle research and cell motility · 2007Review
- Nonmuscle myosin, force maintenance, and the tonic contractile phenotype in smooth muscle.Pflugers Archiv : European journal of physiology · 2006Article
- Phosphorylation of a single head of smooth muscle myosin activates the whole molecule.Biochemistry · 2006Article
- The latch-bridge hypothesis of smooth muscle contraction.Canadian journal of physiology and pharmacology · 2005Review
- Smooth muscle myosin: regulation and properties.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.Biophysical journal · 2004Article
- Nonmuscle Myosin motor of smooth muscle.The Journal of general physiology · 2003Article
- An in vitro assay reveals essential protein components for the "catch" state of invertebrate smooth muscle.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Photolytic release of MgADP reduces rigor force in smooth muscle.Biophysical journal · 2001Article
- The myosin cross-bridge cycle and its control by twitchin phosphorylation in catch muscle.Biophysical journal · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Tonic rabbit femoral artery and phasic rabbit ileum smooth muscles permeabilized with Triton X-100 were activated either by increasing [Ca2+] from pCa > 8.0 to pCa 6.0 (calcium-ascending protocol) or contracted at pCa 6.0 before lowering [Ca2+] (calcium-descending protocol). The effects of, respectively, high [MgATP]/low [MgADP] [10 mM MgATP + creatine phosphate (CP) + creatine kinase (CK)] or low [MgATP]/[MgADP] (2 mM MgATP, 0 CP, 0 CK) on the "force-[Ca]" relationships were determined. In femoral artery at low, but not at high, [MgATP]/[MgADP] the force and the ratio of stiffness/force at pCa 7.2 were significantly higher under the calcium-descending than calcium-ascending protocols (54% vs. 3% of Po, the force at pCa 6.0) (force hysteresis); the levels of regulatory myosin light chain (MLC20) phosphorylation (9 +/- 2% vs. 10 +/- 2%) and the velocities of unloaded shortening V0 (0.02 +/- 0.004 l/s with both protocols) were not significantly different. No significant force hysteresis was detected in rabbit ileum under either of these experimental conditions. [MgADP], measured in extracts of permeabilized femoral artery strips by two methods, was 130-140 microM during maintained force under the calcium-descending protocol. Exogenous CP (10 mM) applied during the descending protocol reduced endogenous [MgADP] to 46 +/- 10 microM and abolished force hysteresis: residual force at low [Ca2+] was 17 +/- 5% of maximal force. We conclude that the proportion of force-generating nonphosphorylated (AMdp) relative to phosphorylated cross-bridges is higher on the Ca2+-descending than on the Ca2+-ascending force curve in tonic smooth muscle, that this population of positively strained dephosphorylated cross-bridges has a high affinity for MgADP, and that the dephosphorylated AMdp . MgADP state makes a significant contribution to force maintenance at low levels of MLC20 phosphorylation.
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