ArticleBiophysical journal1998
Intracellular fluorescent probe concentrations by confocal microscopy.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 111 citations in OpenAlex.
- From Microscopy to Nanoscopy: Contemporary Physical Methods in Mitochondrial Structural Biology.International journal of molecular sciences · 2026Review
- Biomolecular Phase Boundaries are Described by a Solubility Product That Accounts for Variable Stoichiometry and Soluble Oligomers.Journal of the American Chemical Society · 2025Article
- Biomolecular phase boundaries are described by a solubility product that accounts for variable stoichiometry and soluble oligomers.bioRxiv : the preprint server for biology · 2025Article
- Intraneuronal Aβ accumulation causes tau hyperphosphorylation via endolysosomal leakage.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Measurement of solubility product reveals the interplay of oligomerization and self-association for defining condensate formation.Molecular biology of the cell · 2024Article
- Measurement of solubility product in a model condensate reveals the interplay of small oligomerization and self-association.bioRxiv : the preprint server for biology · 2024Article
- Biomedical Applications of Translational Optical Imaging: From Molecules to Humans.Molecules (Basel, Switzerland) · 2021Review
- The solubility product extends the buffering concept to heterotypic biomolecular condensates.eLife · 2021Article
- A quantitative inventory of yeast P body proteins reveals principles of composition and specificity.eLife · 2020Article
- Dynamics of the mitochondrial permeability transition pore: Transient and permanent opening events.Archives of biochemistry and biophysics · 2019Article
- Methods for Physical Characterization of Phase-Separated Bodies and Membrane-less Organelles.Journal of molecular biology · 2018Review
- Angiotensin II-induced hypertension blunts thick ascending limb NO production by reducing NO synthase 3 expression and enhancing threonine 495 phosphorylation.American journal of physiology. Renal physiology · 2015Article
- Biophysical significance of the inner mitochondrial membrane structure on the electrochemical potential of mitochondria.Physical review. E, Statistical, nonlinear, and soft matter physics · 2013Article
- Photoexcitation of tumor-targeted corroles induces singlet oxygen-mediated augmentation of cytotoxicity.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
- Quantitative measurement of mitochondrial membrane potential in cultured cells: calcium-induced de- and hyperpolarization of neuronal mitochondria.The Journal of physiology · 2012Article
- Stoichiometry of Nck-dependent actin polymerization in living cells.The Journal of cell biology · 2012Article
- Investigating photoexcitation-induced mitochondrial damage by chemotherapeutic corroles using multimode optical imaging.Journal of biomedical optics · 2012Article
- The mitochondrial Ca2+ uniporter MCU is essential for glucose-induced ATP increases in pancreatic β-cells.PloS one · 2012Article
- Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies.Biomarkers in medicine · 2010Review
- Membrane trafficking in guard cells during stomatal movement: Application of an image processing technique.Plant signaling & behavior · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A general method is described that takes advantage of the optical sectioning properties of a confocal microscope to enable measurement of both absolute and relative concentrations of fluorescent molecules inside cells. For compartments within cells that are substantially larger than the point spread function, the fluorescence intensity is simply proportional to the concentration of the fluorophore. For small compartments, the fluorescence intensity is diluted by contributions from regions outside the compartment. Corrections for this dilution can be estimated via calibrations that are based on the intensity distribution found in a computationally synthesized model for a cell or organelle that has been blurred by convolution with the microscope point spread function. The method is illustrated with four test cases: estimation of intracellular concentration of a fluorescent calcium indicator; estimation of the relative distribution between the neurite and soma of a neuronal cell of the InsP3 receptor on the endoplasmic reticulum; estimation of the distribution of the bradykinin receptor along the surface of a neuronal cell; and relative distribution of a potentiometric dye between the mitochondria and cytosol as a means of assaying mitochondrial membrane potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.