ArticleJournal of muscle research and cell motility1998
Generation of functional beta-actinin (CapZ) in an E. coli expression system.
Article in Journal of muscle research and cell motility, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 64 citations in OpenAlex.
- Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin.Cytoskeleton (Hoboken, N.J.) · 2025Article
- A unified purification method for actin-binding proteins using a TEV-cleavable His-Strep-tag.MethodsX · 2024Article
- Coordination of actin plus-end dynamics by IQGAP1, formin, and capping protein.The Journal of cell biology · 2024Article
- Coordination of actin plus-end dynamics by IQGAP1, formin, and capping protein.bioRxiv : the preprint server for biology · 2024Article
- Single-molecule imaging of IQGAP1 regulating actin filament dynamics.Molecular biology of the cell · 2022Article
- The structure of the actin filament uncapping complex mediated by twinfilin.Science advances · 2021Article
- EB1 Directly Regulates APC-Mediated Actin Nucleation.Current biology : CB · 2020Article
- Force-history dependence and cyclic mechanical reinforcement of actin filaments at the single molecular level.Journal of cell science · 2019Article
- Article
- Heterodimeric capping protein is required for stereocilia length and width regulation.The Journal of cell biology · 2017Article
- Adaptive Response of Actin Bundles under Mechanical Stress.Biophysical journal · 2017Article
- Adenomatous polyposis coli nucleates actin assembly to drive cell migration and microtubule-induced focal adhesion turnover.The Journal of cell biology · 2017Article
- Regulation of actin catch-slip bonds with a RhoA-formin module.Scientific reports · 2016Article
- Single-molecule visualization of a formin-capping protein 'decision complex' at the actin filament barbed end.Nature communications · 2015Article
- Pivotal and distinct role for Plasmodium actin capping protein alpha during blood infection of the malaria parasite.Molecular microbiology · 2015Article
- The F-BAR protein Hof1 tunes formin activity to sculpt actin cables during polarized growth.Molecular biology of the cell · 2014Article
- Subunits of the Drosophila actin-capping protein heterodimer regulate each other at multiple levels.PloS one · 2014Article
- Nonlinear cross-bridge elasticity and post-power-stroke events in fast skeletal muscle actomyosin.Biophysical journal · 2013Article
- Organization of F-actin by Fesselin (avian smooth muscle synaptopodin 2).Biochemistry · 2013Article
- ATP-dependent regulation of actin monomer-filament equilibrium by cyclase-associated protein and ADF/cofilin.The Biochemical journal · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
beta-actinin (CapZ) is a heterodimeric actin-binding protein which caps the barbed end of action filaments and nucleates actin-polymerization in a Ca2+ -independent manner. In myofibrils it is localized in the Z-lines. As judged by these properties of b-actinin, it is conceivable that beta-actinin is involved in the regulation of actin assembly, especially in the formation of I-Z-I complex during myofribrillogenesis. In this study, we devised a system to produce functional beta-actinin in E. Coli. The cDNAs of beta I' and beta II subunits of beta-actinin were obtained by RT-PCR methods using the published sequence as references, and subcloned in a pET vector. When the proteins were produced with the cDNA of either beta I' and beta II in E. coli, the proteins were insoluble and non-functional. However, when the cDNAs encoding the two subunits were cloned into a single vector and both proteins were expressed simultaneously, the proteins became soluble and purified as a functional heterodimer The activity of the purified proteins was not distinguishable from that of beta-actinin purified from skeletal muscle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.