ArticleMolecular and cellular biology1998
Mechanistic principles in NR box-dependent interaction between nuclear hormone receptors and the coactivator TIF2.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 106 citations in OpenAlex.
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- SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARα.Cell reports · 2019Article
- Molecular Functions of Thyroid Hormone Signaling in Regulation of Cancer Progression and Anti-Apoptosis.International journal of molecular sciences · 2019Review
- Clathrin Heavy Chain Interacts With Estrogen Receptor α and Modulates 17β-Estradiol Signaling.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Role of Nuclear Receptors in Central Nervous System Development and Associated Diseases.Journal of experimental neuroscience · 2015Review
- Comparative molecular profiling of the PPARα/γ activator aleglitazar: PPAR selectivity, activity and interaction with cofactors.ChemMedChem · 2012Article
- The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.The Journal of biological chemistry · 2009Article
- Nutritional flavonoids impact on nuclear and extranuclear estrogen receptor activities.Genes & nutrition · 2006Article
- Aryl hydrocarbon receptor-mediated transcription: ligand-dependent recruitment of estrogen receptor alpha to 2,3,7,8-tetrachlorodibenzo-p-dioxin-responsive promoters.Molecular and cellular biology · 2005Article
- Progesterone and glucocorticoid receptors recruit distinct coactivator complexes and promote distinct patterns of local chromatin modification.Molecular and cellular biology · 2003Article
- Microtubule-dependent subcellular redistribution of the transcriptional coactivator p/CIP.Molecular and cellular biology · 2002Article
- A structural model of the constitutive androstane receptor defines novel interactions that mediate ligand-independent activity.Molecular and cellular biology · 2002Article
- A transcriptional inhibitor targeted by the atypical orphan nuclear receptor SHP.EMBO reports · 2002Article
- The pro-apoptotic protein death-associated protein 3 (DAP3) interacts with the glucocorticoid receptor and affects the receptor function.The Biochemical journal · 2000Article
- A tissue-specific coactivator of steroid receptors, identified in a functional genetic screen.Molecular and cellular biology · 2000Article
- The orphan nuclear receptor SHP utilizes conserved LXXLL-related motifs for interactions with ligand-activated estrogen receptors.Molecular and cellular biology · 2000Article
- The androgen receptor amino-terminal domain plays a key role in p160 coactivator-stimulated gene transcription.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear hormone receptors exert transcriptional activation of target genes upon hormone induction via interactions with the basal transcription machinery. This interaction is mediated by cofactors which physically bind to receptors, thereby acting as coactivators or corepressors leading to activation or repression, respectively. Here we report the screening for and cloning of a peroxisome proliferator receptor-interacting protein, the rat homolog of TIF2. By sequence comparison with the related coactivator SRC-1, we identified three short conserved motifs (NR boxes) in both proteins which are the putative binding sites of TIF2 to nuclear hormone receptors. We demonstrate here by generation of amino acid exchanges within the NR boxes that all three boxes located in the receptor interaction domain of TIF2 are necessary and sufficient for interaction. The three boxes individually can bind to hormone receptors but display preferences in binding for certain receptors. In addition, we show that the interaction domain of TIF2 can compete with other AF-2-dependent cofactors for binding to receptors. Finally, we demonstrate cooperative binding of two TIF2 molecules to a heterodimeric nuclear receptor complex even in the presence of only one cognate ligand, indicating an allosteric effect on the heterodimeric partner upon coactivator binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.