ArticleMolecular and cellular biology1998
Dominant-negative mutations in the G-protein-coupled alpha-factor receptor map to the extracellular ends of the transmembrane segments.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast α-Factor Receptor.Biomolecules · 2022Review
- Article
- Oligomerization of yeast α-factor receptor detected by fluorescent energy transfer between ligands.Biophysical journal · 2021Article
- Yeast GPCR signaling reflects the fraction of occupied receptors, not the number.Molecular systems biology · 2016Article
- Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.The Journal of biological chemistry · 2016Article
- Analysis of random PCR-originated mutants of the yeast Ste2 and Ste3 receptors.MicrobiologyOpen · 2016Article
- Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis.Biology open · 2014Article
- Functional and physical interactions among Saccharomyces cerevisiae α-factor receptors.Eukaryotic cell · 2012Article
- The role of pheromone receptors for communication and mating in Hypocrea jecorina (Trichoderma reesei).Fungal genetics and biology : FG & B · 2012Article
- Multiple regulatory roles of the carboxy terminus of Ste2p a yeast GPCR.Pharmacological research · 2012Article
- Comparative NMR analysis of an 80-residue G protein-coupled receptor fragment in two membrane mimetic environments.Biochimica et biophysica acta · 2011Article
- Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p.Journal of molecular biology · 2011Article
- Strategies for isolating constitutively active and dominant-negative pheromone receptor mutants in yeast.Methods in enzymology · 2010Article
- Cross-linking of a DOPA-containing peptide ligand into its G protein-coupled receptor.Biochemistry · 2009Article
- Magnificent seven: roles of G protein-coupled receptors in extracellular sensing in fungi.FEMS microbiology reviews · 2008Review
- Semidominant mutations in reduced epidermal fluorescence 4 reduce phenylpropanoid content in Arabidopsis.Genetics · 2008Article
- Role of extracellular charged amino acids in the yeast alpha-factor receptor.Biochimica et biophysica acta · 2007Article
- Identification of an N-acetylglucosamine transporter that mediates hyphal induction in Candida albicans.Molecular biology of the cell · 2007Article
- Secretin receptor oligomers form intracellularly during maturation through receptor core domains.Biochemistry · 2006Article
- Comparison of class A and D G protein-coupled receptors: common features in structure and activation.Biochemistry · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
G-protein-coupled receptors (GPCRs) transduce the signals for a wide range of hormonal and sensory stimuli by activating a heterotrimeric guanine nucleotide-binding protein (G protein). The analysis of loss-of-function and constitutively active receptor mutants has helped to reveal the functional properties of GPCRs and their role in human diseases. Here we describe the identification of a new class of mutants, dominant-negative mutants, for the yeast G-protein-coupled alpha-factor receptor (Ste2p). Sixteen dominant-negative receptor mutants were isolated based on their ability to inhibit the response to mating pheromone in cells that also express wild-type receptors. Detailed analysis of two of the strongest mutant receptors showed that, unlike other GPCR interfering mutants, they were properly localized at the plasma membrane and did not alter the stability or localization of wild-type receptors. Furthermore, their dominant-negative effect was inversely proportional to the relative amount of wild-type receptors and was reversed by overexpressing the G-protein subunits, suggesting that these mutants compete with the wild-type receptors for the G protein. Interestingly, the dominant-negative mutations are all located at the extracellular ends of the transmembrane segments, defining a novel region of the receptor that is important for receptor signaling. Altogether, our results identify residues of the alpha-factor receptor specifically involved in ligand binding and receptor activation and define a new mechanism by which GPCRs can be inactivated that has important implications for the evaluation of receptor mutations in other G-protein-coupled receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.