ArticleMolecular and cellular biology1998
A sequence of the CIS gene promoter interacts preferentially with two associated STAT5A dimers: a distinct biochemical difference between STAT5A and STAT5B.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 158 citations in OpenAlex.
- Role of the JAK-STAT Pathway in Bovine Mastitis and Milk Production.Animals : an open access journal from MDPI · 2020Review
- Ikaros antagonizes DNA binding by STAT5 in pre-B cells.PloS one · 2020Article
- A Framework of All Discovered Immunological Pathways and Their Roles for Four Specific Types of Pathogens and Hypersensitivities.Frontiers in immunology · 2020Review
- Direct targets of pSTAT5 signalling in erythropoiesis.PloS one · 2017Article
- Lineage-Specific and Non-specific Cytokine-Sensing Genes Respond Differentially to the Master Regulator STAT5.Cell reports · 2016Article
- GH/STAT5 signaling during the growth period in livers of mice overexpressing GH.Journal of molecular endocrinology · 2015Article
- DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking.PloS one · 2014Article
- Gene expression in skeletal muscle after an acute intravenous GH bolus in human subjects: identification of a mechanism regulating ANGPTL4.Journal of lipid research · 2013Article
- SOCS proteins in development and disease.American journal of clinical and experimental immunology · 2013Article
- Respiratory syncytial virus induces functional thymic stromal lymphopoietin receptor in airway epithelial cells.Journal of inflammation research · 2013Article
- Critical Role of STAT5 transcription factor tetramerization for cytokine responses and normal immune function.Immunity · 2012Article
- Mammary gland growth factors: roles in normal development and in cancer.Cold Spring Harbor perspectives in biology · 2010Review
- Distinct alterations in chromatin organization of the two IGF-I promoters precede growth hormone-induced activation of IGF-I gene transcription.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Intrinsic sex differences in the early growth hormone responsiveness of sex-specific genes in mouse liver.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Growth hormone-activated STAT5 may indirectly stimulate IGF-I gene transcription through HNF-3{gamma}.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Dynamic in vivo binding of STAT5 to growth hormone-regulated genes in intact rat liver. Sex-specific binding at low- but not high-affinity STAT5 sites.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Sex-specific early growth hormone response genes in rat liver.Molecular endocrinology (Baltimore, Md.) · 2008Article
- In vivo identification of novel STAT5 target genes.Nucleic acids research · 2008Article
- The different functions of Stat5 and chromatin alteration through Stat5 proteins.Frontiers in bioscience : a journal and virtual library · 2008Review
- Growth hormone regulation of insulin-like growth factor-I gene expression may be mediated by multiple distal signal transducer and activator of transcription 5 binding sites.Endocrinology · 2008Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Two distinct genes encode the closely related signal transducer and activator of transcription proteins STAT5A and STAT5B. The molecular mechanisms of gene regulation by STAT5 and, particularly, the requirement for both STAT5 isoforms are still undetermined. Only a few STAT5 target genes, among them the CIS (cytokine-inducible SH2-containing protein) gene, have been identified. We cloned the human CIS gene and studied the human CIS gene promoter. This promoter contains four STAT binding elements organized in two pairs. By electrophoretic mobility shift assay studies using nuclear extracts of UT7 cells stimulated with erythropoietin, we showed that these four sequences bound to STAT5-containing complexes that exhibited different patterns and affinities: the three upstream STAT binding sequences bound to two distinct STAT5-containing complexes (C0 and C1) and the downstream STAT box bound only to the slower-migrating C1 band. Using nuclear extracts from COS-7 cells transfected with expression vectors for the prolactin receptor, STAT5A, and/or STAT5B, we showed that the C1 complex was composed of a STAT5 tetramer and was dependent on the presence of STAT5A. STAT5B lacked this property and bound with a stronger affinity than did STAT5A to the four STAT sequences as a homodimer (C0 complex). This distinct biochemical difference between STAT5A and STAT5B was confirmed with purified activated STAT5 recombinant proteins. Moreover, we showed that the presence on the same side of the DNA helix of a second STAT sequence increased STAT5 binding and that only half of the palindromic STAT binding sequence was sufficient for the formation of a STAT5 tetramer. Again, STAT5A was essential for this cooperative tetrameric association. This property distinguishes STAT5A from STAT5B and could be essential to explain the transcriptional regulation diversity of STAT5.
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