ArticleMolecular and cellular biology1998
Requirements for chromatin modulation and transcription activation by the Pho4 acidic activation domain.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.Nature cell biology · 2026Article
- Divergence in a eukaryotic transcription factor's co-TF dependence involves multiple intrinsically disordered regions.Nature communications · 2025Article
- Divergence in a Eukaryotic Transcription Factor's co-TF Dependence Involves Multiple Intrinsically Disordered Regions.bioRxiv : the preprint server for biology · 2025Article
- A Twist-Box domain of the C. elegans Twist homolog, HLH-8, plays a complex role in transcriptional regulation.Genetics · 2023Article
- Nucleosome Remodeling at the YeastInternational journal of molecular sciences · 2023Article
- Computational studies on Begomoviral AC2/C2 proteins.Bioinformation · 2018Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- Article
- A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.The Journal of biological chemistry · 2010Article
- p65 Negatively regulates transcription of the cyclin E gene.The Journal of biological chemistry · 2010Article
- Nucleosome retention and the stochastic nature of promoter chromatin remodeling for transcription.Cell · 2008Article
- Genomic analysis of PIS1 gene expression.Eukaryotic cell · 2005Article
- GM-CSF promoter chromatin remodelling and gene transcription display distinct signal and transcription factor requirements.Nucleic acids research · 2005Article
- Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes.PLoS biology · 2003Article
- Transcription activator interactions with multiple SWI/SNF subunits.Molecular and cellular biology · 2002Article
- Molecular distinction between specification and differentiation in the myogenic basic helix-loop-helix transcription factor family.Molecular and cellular biology · 2001Article
- A ligand binding domain mutation in the mouse glucocorticoid receptor functionally links chromatin remodeling and transcription initiation.Molecular and cellular biology · 1999Article
- Structural and functional cross-talk between a distant enhancer and the epsilon-globin gene promoter shows interdependence of the two elements in chromatin.Molecular and cellular biology · 1999Article
- An activation-specific role for transcription factor TFIIB in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Perhaps the best characterized example of an activator-induced chromatin transition is found in the activation of the Saccharomyces cerevisiae acid phosphatase gene PHO5 by the basic helix-loop-helix (bHLH) transcription factor Pho4. Transcription activation of the PHO5 promoter by Pho4 is accompanied by the remodeling of four positioned nucleosomes which is dependent on the Pho4 activation domain but independent of transcription initiation. Whether the requirements for transcription activation through the TATA sequence are different from those necessary for the chromatin transition remains a major outstanding question. In an attempt to understand better the ability of Pho4 to activate transcription and to remodel chromatin, we have initiated a detailed characterization of the Pho4 activation domain. Using both deletion and point mutational analysis, we have defined residues between positions 75 and 99 as being both essential and sufficient to mediate transcription activation. Significantly, there is a marked concordance between the ability of mutations in the Pho4 activation domain to induce chromatin opening and transcription activation. Interestingly, the requirements for transcription activation within the Pho4 activation domain differ significantly if fused to a heterologous bHLH-leucine zipper DNA-binding domain. The implications for transcription activation by Pho4 are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.