ArticleMolecular and cellular biology1998
I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Halting Recombinant Adeno-Associated Virus Transgene Expression Using mRNA-Lipid Nanoparticle-Delivered Meganucleases.Human gene therapy · 2025Article
- HEHR: Homing Endonuclease-Mediated Homologous Recombination for Efficient Adenovirus Genome Engineering.Genes · 2022Article
- Modeling in yeast how rDNA introns slow growth and increase desiccation tolerance in lichens.G3 (Bethesda, Md.) · 2021Article
- Histone H2A insufficiency causes chromosomal segregation defects due to anaphase chromosome bridge formation at rDNA repeats in fission yeast.Scientific reports · 2019Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- RNA aptamers that functionally interact with green fluorescent protein and its derivatives.Nucleic acids research · 2012Article
- Tracking chromosome dynamics in live yeast cells: coordinated movement of rDNA homologs and anaphase disassembly of the nucleolus during meiosis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Knocking down gene function with an RNA aptamer expressed as part of an intron.Nucleic acids research · 2010Article
- Article
- Evolution of Pleopsidium (lichenized Ascomycota) S943 group I introns and the phylogeography of an intron-encoded putative homing endonuclease.Journal of molecular evolution · 2007Article
- Self-splicing of a group I intron reveals partitioning of native and misfolded RNA populations in yeast.RNA (New York, N.Y.) · 2006Article
- Flanking sequences with an essential role in hydrolysis of a self-cleaving group I-like ribozyme.Nucleic acids research · 2000Article
- Pentamidine inhibition of group I intron splicing in Candida albicans correlates with growth inhibition.Antimicrobial agents and chemotherapy · 2000Article
- Expression of the Naegleria intron endonuclease is dependent on a functional group I self-cleaving ribozyme.RNA (New York, N.Y.) · 2000Article
- Survey and summary: transcription by RNA polymerases I and III.Nucleic acids research · 2000Review
- Functional alpha-fragment of beta-galactosidase can be expressed from the mobile group I intron PpLSU3 embedded in yeast pre-ribosomal RNA derived from the chromosomal rDNA locus.Nucleic acids research · 2000Article
- In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.The EMBO journal · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
PpLSU3, a mobile group I intron in the rRNA genes of Physarum polycephalum, also can home into yeast chromosomal ribosomal DNA (rDNA) (D. E. Muscarella and V. M. Vogt, Mol. Cell. Biol. 13:1023-1033, 1993). By integrating PpLSU3 into the rDNA copies of a yeast strain temperature sensitive for RNA polymerase I, we have shown that the I-PpoI homing endonuclease encoded by PpLSU3 is expressed from an RNA polymerase I transcript. We have also developed a method to integrate mutant forms of PpLSU3 as well as the Tetrahymena intron TtLSU1 into rDNA, by expressing I-PpoI in trans. Analysis of I-PpoI expression levels in these mutants, along with subcellular fractionation of intron RNA, strongly suggests that the full-length excised intron RNA, but not RNAs that are further cleaved, serves as or gives rise to the mRNA.
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