ArticleJournal of clinical microbiology1998
Comparison of PCR, nested PCR, and random amplified polymorphic DNA PCR for detection and typing of Ureaplasma urealyticum in specimens from pregnant women.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Intra-amniotic infection withBrain communications · 2025Article
- Distribution ofThe Indian journal of medical research · 2019Article
- Infection-induced thrombin production: a potential novel mechanism for preterm premature rupture of membranes (PPROM).American journal of obstetrics and gynecology · 2018Article
- A case of oropharyngealJMM case reports · 2018Article
- The Human Ureaplasma Species as Causative Agents of Chorioamnionitis.Clinical microbiology reviews · 2017Review
- Article
- Effect of Genital Sampling Site on the Detection and Quantification ofInfectious diseases in obstetrics and gynecology · 2017Article
- Article
- Ureaplasma parvum serovar 3 multiple banded antigen size variation after chronic intra-amniotic infection/colonization.PloS one · 2013Article
- Ureaplasma serovars & their antimicrobial susceptibility in patients of infertility & genital tract infections.The Indian journal of medical research · 2012Article
- Genotypic characterization of ureaplasma serovars from clinical isolates by pulsed-field gel electrophoresis.Journal of clinical microbiology · 2011Article
- The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum.Biology of reproduction · 2010Article
- Detection and characterization of human Ureaplasma species and serovars by real-time PCR.Journal of clinical microbiology · 2010Article
- Pulmonary vascular and alveolar development in preterm lambs chronically colonized with Ureaplasma parvum.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- Experimental amniotic fluid infection in sheep: effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep.American journal of obstetrics and gynecology · 2008Article
- Mycoplasmas and ureaplasmas as neonatal pathogens.Clinical microbiology reviews · 2005Review
- Interleukin-1 receptor antagonist gene polymorphism, vaginal interleukin-1 receptor antagonist concentrations, and vaginal ureaplasma urealyticum colonization in pregnant women.Infection and immunity · 2003Article
- Ureaplasma urealyticum biovar determination in women attending a family planning clinic in Guiné-Bissau, using polymerase chain reaction of the multiple-banded antigen gene.Journal of clinical laboratory analysis · 2002Article
- Simple method for determining biovar and serovar types of Ureaplasma urealyticum clinical isolates using PCR-single-strand conformation polymorphism analysis.Journal of clinical microbiology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum, parvo biovar, mba types 1, 3, and 6, in cultured clinical specimens. The primer pairs were designed by using the polymorphic base positions within a 310- to 311-bp fragment of the 5' end and upstream control region of the mba gene. The specificity of the assay was confirmed with reference serovars 1, 3, 6, and 14 and by the amplified-fragment sizes (81 bp for mba 1, 262 bp for mba 3, and 193 bp for mba 6). A more sensitive nested PCR was also developed. This involved a first-step PCR, using the primers UMS-125 and UMA226, followed by the nested mba-type PCR described above. This nested PCR enabled the detection and typing of small numbers of U. urealyticum cells, including mixtures, directly in original clinical specimens. By using random amplified polymorphic DNA (RAPD) PCR with seven arbitrary primers, we were also able to differentiate the two biovars of U. urealyticum and to identify 13 RAPD-PCR subtypes. By applying these subtyping techniques to clinical samples collected from pregnant women, we established that (i) U. urealyticum is often a persistent colonizer of the lower genital tract from early midtrimester until the third trimester of pregnancy, (ii) mba type 6 was isolated significantly more often (P = 0.048) from women who delivered preterm than from women who delivered at term, (iii) no particular ureaplasma subtype(s) was associated with placental infections and/or adverse pregnancy outcomes, and (iv) the ureaplasma subtypes most frequently isolated from women were the same subtypes most often isolated from infected placentas.
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