ArticleJournal of clinical microbiology1998
Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
70 citing papers in PubMed, 203 citations in OpenAlex.
- Controlled clinical comparison of BacT/ALERT standard aerobic and standard anaerobic blood culture bottles inoculated directly or after transport in sodium polyanethol sulfonate tubes.Journal of clinical microbiology · 2007Trial
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- Rapid Nanopore Sequencing of Positive Blood Cultures Using Automated Benzyl-Alcohol Extraction Improves Time-Critical Sepsis Management.Antibiotics (Basel, Switzerland) · 2025Article
- Optimized Method for Bacterial Nucleic Acid Extraction from Positive Blood Culture Broth for Whole-Genome Sequencing, Resistance Phenotype Prediction, and Downstream Molecular Applications.Journal of clinical microbiology · 2022Article
- BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for CapturingInternational journal of molecular sciences · 2021Article
- Direct Detection ofPathogens (Basel, Switzerland) · 2021Article
- An Isothermal Molecular Point of Care Testing for African Swine Fever Virus Using Recombinase-Aided Amplification and Lateral Flow Assay Without the Need to Extract Nucleic Acids in Blood.Frontiers in cellular and infection microbiology · 2021Article
- Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection.Scientific reports · 2019Article
- Article
- Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers.Scientific reports · 2018Article
- Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology.Scientific reports · 2018Article
- Detection of bacterial pathogens from clinical specimens using conventional microbial culture and 16S metagenomics: a comparative study.BMC infectious diseases · 2017Article
- Improving detection of avian malaria from host blood: a step towards a standardised protocol for diagnostics.Parasitology research · 2016Article
- Benefits of Adding a Rapid PCR-Based Blood Culture Identification Panel to an Established Antimicrobial Stewardship Program.Journal of clinical microbiology · 2016Article
- PCR Conditions for 16S Primers for Analysis of Microbes in the Colon of Rats.Journal of biomolecular techniques : JBT · 2016Article
- Comparison of methods for the identification of microorganisms isolated from blood cultures.Annals of clinical microbiology and antimicrobials · 2016Article
- A population-based study of aerococcal bacteraemia in the MALDI-TOF MS-era.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- The development and application of a molecular community profiling strategy to identify polymicrobial bacterial DNA in the whole blood of septic patients.BMC microbiology · 2015Article
- Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus.Diagnostic microbiology and infectious disease · 2015Article
- A novel technique for detecting antibiotic-resistant typhoid from rapid diagnostic tests.Journal of clinical microbiology · 2015Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Molecular methods are increasingly used to identify microbes in clinical samples. A common technical problem with PCR is failed amplification due to the presence of PCR inhibitors. Initial attempts at amplification of the bacterial 16S rRNA gene from inoculated blood culture media failed for this reason. The inhibitor persisted, despite numerous attempts to purify the DNA, and was identified as sodium polyanetholesulfonate (SPS), a common additive to blood culture media. Like DNA, SPS is a high-molecular-weight polyanion that is soluble in water but insoluble in alcohol. Accordingly, SPS tends to copurify with DNA. An extraction method was designed for purification of DNA from blood culture media and removal of SPS. Blood culture media containing human blood and spiked with Escherichia coli was subjected to an organic extraction procedure with benzyl alcohol, and removal of SPS was documented spectrophotometrically. Successful amplification of the extracted E. coli 16S rRNA gene was achieved by adding 5 microliter of undiluted processed sample DNA to a 50-microliter PCR mixture. When using other purification methods, the inhibitory effect of SPS could be overcome only by dilution of these samples. By our extraction technique, even uninoculated blood culture media were found to contain bacterial DNA when they were subjected to broad-range 16S rRNA gene consensus PCR. We conclude that the blood culture additive SPS is a potent inhibitor of PCR, is resistant to removal by traditional DNA purification methods, but can be removed by a benzyl alcohol extraction protocol that results in improved PCR performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.