ArticleProceedings of the National Academy of Sciences of the United States of America1998
Vesiculation and sorting from PC12-derived endosomes in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Assessing the Potential of NGF-Differentiated PC12 Cells as a Model for Synaptic Transmission.Molecular neurobiology · 2025Article
- Article
- The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.Molecular biology of the cell · 2013Article
- The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.Molecular biology of the cell · 2011Article
- A distinct trans-Golgi network subcompartment for sorting of synaptic and granule proteins in neurons and neuroendocrine cells.Journal of cell science · 2011Article
- Endosomal sorting of readily releasable synaptic vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Endosomal recycling regulates Anthrax Toxin Receptor 1/Tumor Endothelial Marker 8-dependent cell spreading.Experimental cell research · 2010Article
- A fluorescence-based in vitro assay for investigating early endosome dynamics.Nature protocols · 2010Article
- CB(1) cannabinoid receptors and their associated proteins.Current medicinal chemistry · 2010Review
- Sorting in early endosomes reveals connections to docking- and fusion-associated factors.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Roles of BLOC-1 and adaptor protein-3 complexes in cargo sorting to synaptic vesicles.Molecular biology of the cell · 2009Article
- Mint3/X11gamma is an ADP-ribosylation factor-dependent adaptor that regulates the traffic of the Alzheimer's Precursor protein from the trans-Golgi network.Molecular biology of the cell · 2008Article
- Macromolecular-scale resolution in biological fluorescence microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.Molecular biology of the cell · 2005Article
- Biochemical characterization of the coating mechanism of the endosomal donor compartment of synaptic vesicles.Neurochemical research · 2004Article
- The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.Molecular biology of the cell · 2004Article
- The dopamine transporter constitutively internalizes and recycles in a protein kinase C-regulated manner in stably transfected PC12 cell lines.The Journal of biological chemistry · 2003Article
- Ligand-induced internalization of the p75 neurotrophin receptor: a slow route to the signaling endosome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Rab4 regulates formation of synaptic-like microvesicles from early endosomes in PC12 cells.Molecular biology of the cell · 2001Article
- The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Formation of small vesicles resembling synaptic vesicles can be reconstituted in vitro by incubating labeled homogenates of PC12 cells with ATP and two cytoplasmic proteins, AP3 and ARF1 [Faúndez, V., Horng, J.-T. & Kelly, R. B. (1998) Cell 93, 423-432]. To determine whether AP3 was mediating budding from plasma membranes or endosomes the organelle that generated the synaptic vesicles was characterized. The budding activity was enriched in organelles that labeled at 15 degrees C, but not at 4 degrees C, that excluded a marker of plasma membranes and that contained internalized transferrin, indicating that the precursor was an endosome. Vesicles formed from the endosomal precursor in vitro excluded transferrin. We conclude that ARF-mediated vesiculation into synaptic vesicle-sized organelles uses an endosomal precursor and occurs simultaneously in vitro with sorting of synaptic vesicle proteins from other membrane protein constituents of the endosome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.