ArticleThe EMBO journal1998
The Cdc42p effector Gic2p is targeted for ubiquitin-dependent degradation by the SCFGrr1 complex.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 92 citations in OpenAlex.
- Dot6/Tod6 degradation fine-tunes the repression of ribosome biogenesis under nutrient-limited conditions.iScience · 2022Article
- Regulation of Cdc42 for polarized growth in budding yeast.Microbial cell (Graz, Austria) · 2020Review
- Regulation of intrinsic polarity establishment by a differentiation-type MAPK pathway inJournal of cell science · 2020Article
- Article
- Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.Nature methods · 2013Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Morphogenesis and the cell cycle.Genetics · 2012Review
- Article
- Septins: molecular partitioning and the generation of cellular asymmetry.Cell division · 2009Article
- Lessons from fungal F-box proteins.Eukaryotic cell · 2009Review
- Regulation of Gic2 localization and function by phosphatidylinositol 4,5-bisphosphate during the establishment of cell polarity in budding yeast.The Journal of biological chemistry · 2008Article
- Identification of novel membrane-binding domains in multiple yeast Cdc42 effectors.Molecular biology of the cell · 2007Article
- Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.The EMBO journal · 2007Article
- Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle.Molecular biology of the cell · 2007Article
- Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.Eukaryotic cell · 2007Article
- Role of a Cdc42p effector pathway in recruitment of the yeast septins to the presumptive bud site.Molecular biology of the cell · 2006Article
- Glucose signaling in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006Review
- Transcriptional compensation for gene loss plays a minor role in maintaining genetic robustness in Saccharomyces cerevisiae.Genetics · 2005Article
- Degradation of Hof1 by SCF(Grr1) is important for actomyosin contraction during cytokinesis in yeast.The EMBO journal · 2005Article
- Glucose inhibits meiotic DNA replication through SCFGrr1p-dependent destruction of Ime2p kinase.Molecular and cellular biology · 2005Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cdc42p, a Rho-related GTP-binding protein, regulates cytoskeletal polarization and rearrangements in eukaryotic cells. In yeast, Gic1p and Gic2p are effectors of Cdc42p involved in actin polarization at bud emergence. Gic2p is expressed in a cell cycle-dependent manner and rapidly disappears shortly after bud emergence concomitant with the activation of the G1 cyclin-dependent kinase Cdc28p-Clnp. Here we have shown that the rapid disappearance of Gic2p results from ubiquitin-dependent proteolysis. Biochemical and genetic evidence demonstrates that degradation of Gic2p required the Skp1-cullin-F-box protein complex (SCF) components Cdc34p, Cdc53p, Skp1p and Grr1p, but not Cdc4p. Phosphorylation of several C-terminal sites of Gic2p served as part of the recognition signal for ubiquitination. In addition, binding of Gic2p to Cdc42p was a prerequisite for degradation, suggesting that specifically the active form of Gic2p is targeted for destruction. Finally, our data indicate that degradation of Gic2p may be part of a mechanism which restricts cytoskeletal polarization in the G1 phase of the cell cycle.
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