ArticleAntimicrobial agents and chemotherapy1998
Fungal beta-tubulin, expressed as a fusion protein, binds benzimidazole and phenylcarbamate fungicides.
Article in Antimicrobial agents and chemotherapy, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 65 citations in OpenAlex.
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- Fungicide Activity of Culture Extract fromJournal of fungi (Basel, Switzerland) · 2022Article
- Molecular Mechanisms Underlying Fungicide Resistance in Citrus Postharvest Green Mold.Journal of fungi (Basel, Switzerland) · 2021Review
- The ASK1 gene regulates the sensitivity of Fusarium graminearum to carbendazim, conidiation and sexual production by combining with βCurrent genetics · 2021Article
- Effect of Long-Term Fungicide Applications on Virulence and Diversity ofPlants (Basel, Switzerland) · 2019Article
- sRNA Profiling Combined With Gene Function Analysis Reveals a Lack of Evidence for Cross-Kingdom RNAi in the Wheat -Frontiers in plant science · 2019Article
- Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance.Scientific reports · 2018Article
- Antifungal and anthelmintic activity of novel benzofuran derivatives containing thiazolo benzimidazole nucleus: an in vitro evaluation.Journal of chemical biology · 2017Article
- Insights from the genome of Ophiocordyceps polyrhachis-furcata to pathogenicity and host specificity in insect fungi.BMC genomics · 2015Article
- Molecular evolution and functional divergence of tubulin superfamily in the fungal tree of life.Scientific reports · 2014Article
- Seven benzimidazole pesticides combined at sub-threshold levels induce micronuclei in vitro.Mutagenesis · 2013Article
- A screening assay based on host-pathogen interaction models identifies a set of novel antifungal benzimidazole derivatives.Antimicrobial agents and chemotherapy · 2011Article
- Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchards in California.Applied and environmental microbiology · 2003Article
- Mutations in a beta-tubulin disrupt spindle orientation and microtubule dynamics in the early Caenorhabditis elegans embryo.Molecular biology of the cell · 2003Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Benzimidazoles are important antitubulin agents used in veterinary medicine and plant disease control. Resistance is a practical problem correlated with single amino acid changes in beta-tubulin and is often linked to greater sensitivity to phenylcarbamates. This negative cross-resistance creates opportunities for durable antiresistance strategies. Attempts to understand the molecular basis of benzimidazole resistance have been hampered by the inability to purify tubulin from filamentous fungi. We have overcome some of these problems by expressing beta-tubulin as a fusion with a maltose binding protein. This fusion protein is soluble, and we confirm for the first time using a gel filtration assay that benzimidazoles indeed bind to beta-tubulin. This binding is reduced by the mutation Glu198-->Gly198, which also confers resistance. Binding of phenylcarbamates is the complete opposite, reflecting their biological activity and the negative cross-resistance. This suggests that the fungicide binding sites fold correctly in the fusion protein.
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