ArticleThe Journal of cell biology1998
The cytoplasmic tail of rhodopsin acts as a novel apical sorting signal in polarized MDCK cells.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 147 citations in OpenAlex.
- On the Wrong Track: Alterations of Ciliary Transport in Inherited Retinal Dystrophies.Frontiers in cell and developmental biology · 2021Review
- Type 2β Corticotrophin Releasing Factor Receptor Forms a Heteromeric Complex With Dopamine D1 Receptor in Living Cells.Frontiers in pharmacology · 2019Article
- Trafficking Ion Transporters to the Apical Membrane of Polarized Intestinal Enterocytes.Cold Spring Harbor perspectives in biology · 2018Review
- Review
- Four-dimensional live imaging of apical biosynthetic trafficking reveals a post-Golgi sorting role of apical endosomal intermediates.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A polarized cell model for Chikungunya virus infection: entry and egress of virus occurs at the apical domain of polarized cells.PLoS neglected tropical diseases · 2014Article
- Molecular complexes that direct rhodopsin transport to primary cilia.Progress in retinal and eye research · 2014Review
- Biochemical analysis of a rhodopsin photoactivatable GFP fusion as a model of G-protein coupled receptor transport.Vision research · 2013Article
- The roles of evolutionarily conserved functional modules in cilia-related trafficking.Nature cell biology · 2013Review
- The severe autosomal dominant retinitis pigmentosa rhodopsin mutant Ter349Glu mislocalizes and induces rapid rod cell death.The Journal of biological chemistry · 2013Article
- Signals governing the trafficking and mistrafficking of a ciliary GPCR, rhodopsin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Apical targeting of the P2Y(4) receptor is directed by hydrophobic and basic residues in the cytoplasmic tail.American journal of physiology. Cell physiology · 2013Article
- Live-cell imaging evidence for the ciliary transport of rod photoreceptor opsin by heterotrimeric kinesin-2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Polarized sorting and trafficking in epithelial cells.Cell research · 2012Review
- Role of cysteine residues in cell surface expression of the human riboflavin transporter-2 (hRFT2) in intestinal epithelial cells.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida.Journal of cell science · 2011Article
- The cell biology of vision.The Journal of cell biology · 2010Review
- Filamin a binds to CCR2B and regulates its internalization.PloS one · 2010Article
- Charged residues in the C-terminus of the P2Y1 receptor constitute a basolateral-sorting signal.Journal of cell science · 2010Article
- Ciliary transport of opsin.Advances in experimental medicine and biology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
All basolateral sorting signals described to date reside in the cytoplasmic domain of proteins, whereas apical targeting motifs have been found to be lumenal. In this report, we demonstrate that wild-type rhodopsin is targeted to the apical plasma membrane via the TGN upon expression in polarized epithelial MDCK cells. Truncated rhodopsin with a deletion of 32 COOH-terminal residues shows a nonpolar steady-state distribution. Addition of the COOH-terminal 39 residues of rhodopsin redirects the basolateral membrane protein CD7 to the apical membrane. Fusion of rhodopsin's cytoplasmic tail to a cytosolic protein glutathione S-transferase (GST) also targets this fusion protein (GST-Rho39Tr) to the apical membrane. The targeting of GST-Rho39Tr requires both the terminal 39 amino acids and the palmitoylation membrane anchor signal provided by the rhodopsin sequence. The apical transport of GST-Rho39Tr can be reversibly blocked at the Golgi complex by low temperature and can be altered by brefeldin A treatment. This indicates that the membrane-associated GST-Rho39Tr protein may be sorted along a yet unidentified pathway that is similar to the secretory pathway in polarized MDCK cells. We conclude that the COOH-terminal tail of rhodopsin contains a novel cytoplasmic apical sorting determinant. This finding further indicates that cytoplasmic sorting machinery may exist in MDCK cells for some apically targeted proteins, analogous to that described for basolaterally targeted proteins.
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