ArticleThe Journal of cell biology1998
Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 66 citations in OpenAlex.
- Systematic mapping of rRNA 2'-O methylation during frog development and involvement of the methyltransferase Fibrillarin in eye and craniofacial development in Xenopus laevis.PLoS genetics · 2022Article
- On some structural and evolutionary aspects of rDNA amplification in oogenesis of Trachemys scripta turtles.Cell and tissue research · 2021Article
- Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex.Molecular biology reports · 2018Article
- Nucleation by rRNA Dictates the Precision of Nucleolus Assembly.Current biology : CB · 2016Article
- Ribosomal RNA of Hyacinthus orientalis L. female gametophyte cells before and after fertilization.Planta · 2012Article
- Nucleologenesis in the Caenorhabditis elegans embryo.PloS one · 2012Article
- DNA replication timing is deterministic at the level of chromosomal domains but stochastic at the level of replicons in Xenopus egg extracts.Nucleic acids research · 2008Article
- The nucleolus: a model for the organization of nuclear functions.Histochemistry and cell biology · 2006Review
- Requirement of the protein B23 for nucleolar disassembly induced by the FRGY2a family proteins.The Journal of biological chemistry · 2006Article
- Chromatin decondensation and nuclear reprogramming by nucleoplasmin.Molecular and cellular biology · 2006Article
- Nucleolus: from structure to dynamics.Histochemistry and cell biology · 2006Review
- Tracking the interactions of rRNA processing proteins during nucleolar assembly in living cells.Molecular biology of the cell · 2005Article
- Conventional and nonconventional roles of the nucleolus.International review of cytology · 2002Review
- Nucleolar assembly of the rRNA processing machinery in living cells.The Journal of cell biology · 2001Article
- The dynamics of postmitotic reassembly of the nucleolus.The Journal of cell biology · 2000Article
- Initiation of nucleolar assembly is independent of RNA polymerase I transcription.Molecular biology of the cell · 2000Article
- Rearrangement of chromatin domains during development in Xenopus.Genes & development · 2000Article
- Assembly and functional organization of the nucleolus: ultrastructural analysis of Saccharomyces cerevisiae mutants.Molecular biology of the cell · 2000Article
- Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing.The Journal of cell biology · 2000Article
- The ribosomal RNA processing machinery is recruited to the nucleolar domain before RNA polymerase I during Xenopus laevis development.The Journal of cell biology · 2000Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the early development of Xenopus laevis, we followed in individual nuclei the formation of a nucleolus by examining simultaneously its structural organization and its transcriptional competence. Three distinct situations were encountered with different frequencies during development. During the first period of general transcriptional quiescence, the transcription factor UBF of maternal origin, was present in most nuclei at the ribosomal gene loci. In contrast, fibrillarin, a major protein of the processing machinery, was found in multiple prenucleolar bodies (PNBs) whereas nucleolin was dispersed largely in the nucleoplasm. During the second period, for most nuclei these PNBs had fused into two domains where nucleolin concentrated, generating a structure with most features expected from a transcriptionally competent nucleolus. However, RNA polymerase I-dependent transcription was not detected using run-on in situ assays whereas unprocessed ribosomal RNAs were observed. These RNAs were found to derive from a maternal pool. Later, during a third period, an increasing fraction of the nuclei presented RNA polymerase I-dependent transcription. Thus, the structural organization of the nucleolus preceded its transcriptional competence. We conclude that during the early development of X. laevis, the organization of a defined nucleolar structure, is not associated with the transcription process per se but rather with the presence of unprocessed ribosomal RNAs.
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