ArticleGenes & development1998
Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3'-end formation and excision of the 3'-terminal intron.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- The regulation and function of post-transcriptional RNA splicing.Nature reviews. Genetics · 2025Review
- The Role of RNA-Binding Proteins in Hematological Malignancies.International journal of molecular sciences · 2022Review
- Alternative polyadenylation by sequential activation of distal and proximal PolyA sites.Nature structural & molecular biology · 2022Article
- HibeRNAtion: HIV-1 RNA Metabolism and Viral Latency.Frontiers in cellular and infection microbiology · 2022Review
- Transcription of intragenic CpG islands influences spatiotemporal host gene pre-mRNA processing.Nucleic acids research · 2020Article
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA.Nature communications · 2017Article
- Localization of RNAPII and 3' end formation factor CstF subunits on C. elegans genes and operons.Transcription · 2016Article
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- Kinetic competition during the transcription cycle results in stochastic RNA processing.eLife · 2014Article
- The Chironomus tentans genome sequence and the organization of the Balbiani ring genes.BMC genomics · 2014Article
- Coupling mRNA processing with transcription in time and space.Nature reviews. Genetics · 2014Review
- An essential role for Clp1 in assembly of polyadenylation complex CF IA and Pol II transcription termination.Nucleic acids research · 2012Article
- Review
- Updating the CTD Story: From Tail to Epic.Genetics research international · 2011Article
- Regulation of transcription termination in the nematode Caenorhabditis elegans.Nucleic acids research · 2009Article
- "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions.Molecular cell · 2009Review
- Co-transcriptional splicing of constitutive and alternative exons.RNA (New York, N.Y.) · 2009Article
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
- Specific combinations of SR proteins associate with single pre-messenger RNAs in vivo and contribute different functions.The Journal of cell biology · 2009Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed transcription termination, 3'-end formation, and excision of the 3'-terminal intron in vivo in the Balbiani ring 1 (BR1) gene and its pre-mRNA. We show that full-length RNA transcripts are evenly spaced on the gene from a position 300 bp upstream to a region 500-700 bp downstream of the polyadenylation sequence. Very few full-length transcripts and no short, cleaved, nascent transcripts could be observed downstream of this region. Pre-mRNA with 10-20 adenylate residues accumulates at the active gene and then rapidly leaves from the gene locus. Only polyadenylated pre-mRNAs could be detected in the nucleoplasm. Our results are consistent with the hypothesis that transcription termination occurs in a narrow region for the majority of transcripts, simultaneous with 3'-end formation. Excision of the 3'-terminal intron occurs before 3'-end formation in about 5% of the nascent transcripts. When transcription terminates, 3' cleavage takes place and 10-20 adenylate residues are added, the 3'-terminal intron is excised from additionally about 75% of the pre-mRNA at the gene locus. Our data support a close temporal and spatial coupling of transcription termination and the cleavage and initial polyadenylation of 3'-end formation. Excision of the 3'-terminal intron is highly stimulated as the cleavage/polyadenylation complex assembles and 3'-end formation is initiated.
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