ArticleThe Journal of physiology1998
Single channel currents at six microsecond resolution elicited by acetylcholine in mouse myoballs.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- A deep learning approach to real-time Markov modeling of ion channel gating.Communications chemistry · 2024Article
- 2D-dwell-time analysis with simulations of ion-channel gating using high-performance computing.Biophysical journal · 2023Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- In praise of single channel kinetics.The Journal of general physiology · 2016Article
- Measurement of DNA Translocation Dynamics in a Solid-State Nanopore at 100 ns Temporal Resolution.Nano letters · 2016Article
- α-Conotoxin M1 (CTx) blocks αδ binding sites of adult nicotinic receptors while ACh binding at αε sites elicits only small and short quantal synaptic currents.Physiological reports · 2014Article
- Agonists binding nicotinic receptors elicit specific channel-opening patterns at αγ and αδ sites.The Journal of physiology · 2014Article
- Single ion channel recordings with CMOS-anchored lipid membranes.Nano letters · 2013Article
- Strength-duration relationship for extracellular neural stimulation: numerical and analytical models.Journal of neurophysiology · 2010Article
- Interference of shot noise of open-channel current with analysis of fast gating: patchers do not (Yet) have to care.The Journal of membrane biology · 2009Article
- Four-mode gating model of fast inactivation of sodium channel Nav1.2a.Pflugers Archiv : European journal of physiology · 2008Article
- Strengths and limits of Beta distributions as a means of reconstructing the true single-channel current in patch clamp time series with fast gating.The Journal of membrane biology · 2006Article
- The power of two-dimensional dwell-time analysis for model discrimination, temporal resolution, multichannel analysis and level detection.The Journal of membrane biology · 2006Article
- Probing ion-channel pores one proton at a time.Nature · 2005Article
- Short openings in high resolution single channel recordings of mouse nicotinic receptors.The Journal of physiology · 2005Article
- Gating of acetylcholine receptor channels: brownian motion across a broad transition state.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A speed limit for conformational change of an allosteric membrane protein.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- A subsequent fit of time series and amplitude histogram of patch-clamp records reveals rate constants up to 1 per microsecond.The Journal of membrane biology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. A patch-clamp set-up was optimized for low noise and high time resolution. An Axoclamp 200B amplifier was modified to incorporate a Teflon connector to the electrode. An electrode puller was equipped with a hydrogen-oxygen burner to produce quartz-glass pipettes with optimally 0.2 micron openings and 20 MOmega resistance. 2. The r.m.s. (root mean square) noise of sealed pipettes in the bath ranged from 3.6 fA with 100 Hz filter cut-off to 1.5 pA with 61 kHz filter cut-off. At these extremes currents of 17 fA and more than 3 ms, or 9 pA and more than 6 micros could be resolved with a negligible error rate. 3. The system was tested on mouse myoballs, recording 9-10 pA single channel currents on-cell at -200 mV polarization which were elicited by 0.1-5000 microM acetylcholine (ACh). 4. Distributions of open and closed times and of correlations of open times to the preceding closed time defined several open states: single openings with mean durations of 1.2 and 25 micros, from single-liganded receptors, and bursts of 10 ms mean duration containing on average 800 micros openings and 16 micros closings, from double liganded receptors. Above 0.1 mM ACh these openings are interrupted increasingly by on average 18 micros and 72 micros channel blocks by ACh.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.