ArticleApplied and environmental microbiology1998
Genetic characterization and physiological role of endopeptidase O from Lactobacillus helveticus CNRZ32.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Effect of fermentation using different lactic acid bacteria strains on the nutrient components and mineral bioavailability of soybean yogurt alternative.Frontiers in nutrition · 2023Article
- Comparative studies of versatile extracellular proteolytic activities of lactic acid bacteria and their potential for extracellular amino acid productions as feed supplements.Journal of animal science and biotechnology · 2019Article
- Dipeptidase activity and growth of heat-treated commercial dairy starter culture.Applied biochemistry and biotechnology · 2015Article
- Crucial role for insertion sequence elements in Lactobacillus helveticus evolution as revealed by interstrain genomic comparison.Applied and environmental microbiology · 2010Article
- Engineered vaginal lactobacillus strain for mucosal delivery of the human immunodeficiency virus inhibitor cyanovirin-N.Antimicrobial agents and chemotherapy · 2006Article
- Enzymatic ability of Bifidobacterium animalis subsp. lactis to hydrolyze milk proteins: identification and characterization of endopeptidase O.Applied and environmental microbiology · 2005Article
- Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.Applied and environmental microbiology · 2005Article
- Hydrolysis of casein-derived peptides alpha(S1)-casein(f1-9) and beta-casein(f193-209) by Lactobacillus helveticus peptidase deletion mutants indicates the presence of a previously undetected endopeptidase.Applied and environmental microbiology · 2003Article
- Identification and characterization of Lactobacillus helveticus PepO2, an endopeptidase with post-proline specificity.Applied and environmental microbiology · 2003Article
- Cloning and expression of an oligopeptidase, PepO, with novel specificity from Lactobacillus rhamnosus HN001 (DR20).Applied and environmental microbiology · 2002Article
- Gene cloning, sequencing, and inactivation of the branched-chain aminotransferase of Lactococcus lactis LM0230.Applied and environmental microbiology · 2000Article
- Streptococcus parasanguis pepO encodes an endopeptidase with structure and activity similar to those of enzymes that modulate peptide receptor signaling in eukaryotic cells.Infection and immunity · 1999Article
- Genetic characterization of a cell envelope-associated proteinase from Lactobacillus helveticus CNRZ32.Journal of bacteriology · 1999Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A previously identified insert expressing an endopeptidase from a Lactobacillus helveticus CNRZ32 genomic library was characterized. Nucleotide sequence analysis revealed an open reading frame of 1,941 bp encoding a putative protein of 71.2 kDa which contained a zinc-protease motif. Protein homology searches revealed that this enzyme has 40% similarity with endopeptidase O (PepO) from Lactococcus lactis P8-2-47. Northern hybridization revealed that pepO is monocistronic and is expressed throughout the growth phase. CNRZ32 derivatives lacking PepO activity were constructed via gene replacement. Enzyme assays revealed that the PepO mutant had significantly reduced endopeptidase activity when compared to CNRZ32 with two of the three substrates examined. Growth studies indicated that PepO has no detectable effect on growth rate or acid production by Lactobacillus helveticus CNRZ32 in amino acid defined or skim milk medium.
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